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Identify the mutation(s) that lead to the most loss in transcriptional activity, and discuss whether those match expectations based on the consensus sequence for the Initiator. B. Hypothesize a molecular mechanism to explain how the mutations identified in Part A result in loss of transcription activity. C. Identify whether any mutations cause transcription to initiate from a different position than wild-type, and provide a brief explanation as to why that occurs for those mutant core promoters.

Identify the mutation(s) that lead to the most loss in transcriptional activity, and discuss whether those match expectations based on the consensus sequence for the Initiator. B. Hypothesize a molecular mechanism to explain how the mutations identified in Part A result in loss of transcription activity. C. Identify whether any mutations cause transcription to initiate from a different position than wild-type, and provide a brief explanation as to why that occurs for those mutant core promoters.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A. Identify the mutation(s) that lead to the most loss in transcriptional activity, and discuss
whether those match expectations based on the consensus sequence for the Initiator.
B. Hypothesize a molecular mechanism to explain how the mutations identified in Part A
result in loss of transcription activity.
C. Identify whether any mutations cause transcription to initiate from a different position
than wild-type, and provide a brief explanation as to why that occurs for those mutant
core promoters.

THIS IS A PRIMER EXTENSION ASSAY

WT Single Nucleotide Substitutions of the PMAIP1 Promoter A T ст CA GA GT ↓↓↓↓↓↓↓↓↓↓ G C A A TGAGA C -5 -4 -3 -2 -1 +1 +2 +3 +4 +5 Positions Relative to +1 TSS Single Nucleotide Substitutions of the TFRC Promoter CTCA GA G C WT ↓↓↓↓↓↓↓↓↓ ↓ T TAAT G A GA T -5 -4 -3 -2 -1 +1 +2 +3 +4 +5 Positions Relative to +1 TSS
Transcribed Image Text:WT Single Nucleotide Substitutions of the PMAIP1 Promoter A T ст CA GA GT ↓↓↓↓↓↓↓↓↓↓ G C A A TGAGA C -5 -4 -3 -2 -1 +1 +2 +3 +4 +5 Positions Relative to +1 TSS Single Nucleotide Substitutions of the TFRC Promoter CTCA GA G C WT ↓↓↓↓↓↓↓↓↓ ↓ T TAAT G A GA T -5 -4 -3 -2 -1 +1 +2 +3 +4 +5 Positions Relative to +1 TSS
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