Time (h) 1 3 6 12 Mouse thymus cells were treated with 10 micromolar dexamethasone to induce apoptosis. At the indicated times the chromosomal DNA was extracted and analyzed by gel electrophoresis, which separates the DNA fragments based upon size differences. When cells undergo apoptosis their DNA is cut into fragments that are approximately multiples of 200 base pairs. This results in a characteristic “ladder" appearance when the fragments are separated by gel electrophoresis, as shown above. Based upon what you know about eukaryotic chromatin, explain why this pattern of DNA fragments is obtained.
Time (h) 1 3 6 12 Mouse thymus cells were treated with 10 micromolar dexamethasone to induce apoptosis. At the indicated times the chromosomal DNA was extracted and analyzed by gel electrophoresis, which separates the DNA fragments based upon size differences. When cells undergo apoptosis their DNA is cut into fragments that are approximately multiples of 200 base pairs. This results in a characteristic “ladder" appearance when the fragments are separated by gel electrophoresis, as shown above. Based upon what you know about eukaryotic chromatin, explain why this pattern of DNA fragments is obtained.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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0 1 3 6
12
Mouse thymus cells were treated with 10 micromolar dexamethasone to induce apoptosis. At
the indicated times the chromosomal DNA was extracted and analyzed by gel electrophoresis,
which separates the DNA fragments based upon size differences.
When cells undergo apoptosis their DNA is cut into fragments that are approximately
multiples of 200 base pairs. This results in a characteristic “ladder" appearance when the
fragments are separated by gel electrophoresis, as shown above. Based upon what you know
about eukaryotic chromatin, explain why this pattern of DNA fragments is obtained.
6.
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