Please help with 2a) 2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA Polymerase III, that are active during prokaryotic DNA replication. Suppose you generated a mutant E. coli strain in which DNA Polymerase III was inactivated (all its enzymatic activities were non- functional) - assuming that all the other enzymes involved in replication remained fully functional, how would DNA replication in these mutant cells without DNA Pol III differ from DNA replication in normal E. coli? Briefly explain why you would expect to see that change/those changes in DNA replication in the mutant cells.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Please help with 2a)
2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA Polymerase
III, that are active during prokaryotic
strain in which DNA Polymerase III was inactivated (all its enzymatic activities were non-
functional) - assuming that all the other enzymes involved in replication remained fully
functional, how would DNA replication in these mutant cells without DNA Pol III differ from
DNA replication in normal E. coli? Briefly explain why you would expect to see that
change/those changes in DNA replication in the mutant cells.
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