help please For each of the proteins involved in DNA replication in E.coli that is shown, select the best description.H v PrimaseA Adds/subtracts supercoils and can remove knots or loops in DNAÉ v DNA Polymerase I6. is responsible for unwinding dsDNA into SSDNAC. requires ATP or NAD* as a co-substrateD. binds to specific sequence of DNA opposite the ori and inhibits helicaseDNA Polymerase liIDNA ligaseHelicaseE has B subunits that form a ring-like sliding clampSSB proteinsF. has 5'.>3' exonuclease activityG. binds to and stabilizes single-stranded DNATopoisomerasesTusH. synthesizes a short section of RNA on a strand of DNA

Given, Primase Adds/subtracts supercoils and can remove the knots or loops in DNA DNA Polymerase I is responsible for unwinding dsDNA into ssDNA DNA Polymerase III requires ATP or NAD+ as a co-substrate DNA ligase binds to specific sequence of DNA opposite the ori and inhibits helicase Helicase has β subunits that form a ring like sliding clamp SSB proteins has 5'→3' exonuclease activity Topoisomerase binds to and stabilize single stranded DNA. Tus synthesize a short section of RNA on a strand of DNA.Primase - synthesize a short section of RNA on a strand of DNA. A short fragment of RNA is required as a primer for synthesizing new DNA. Primase is a specific RNA polymerase, it synthesize primer on the DNA template. Primers are constantly synthesized on the lagging strand of DNA while leading strand only require almost a single primer. DNA Polymerase I - has 5'→3' exonuclease activity. DNA polymerase I has unique 5'→3' exonuclease activity. This activity helps the enzyme to replace nucleotides from the 5' terminus by nick translation. DNA Polymerase III - has β subunits that form a ring like sliding clamp. β-subunit also called as beta clamp is a part of the holoenzyme of DNA polymerase III. The double stranded DNA in bacteria are encircled by beta-clamp. DNA ligase - requires ATP or NAD+ as a co-substrate. DNA ligase in bacteria uses β-NAD+ as their co-substrate while in other cells it uses ATP. DNA ligase forms phosphodiester linkage between DNA formed by DNA polymerase III and fragment produced by DNA pol I. Energy is provided by breakdown of ATP.Helicase - is responsible for unwinding dsDNA into ssDNA. Helicases move along the DNA helix and unwinds the strand. SSB proteins - binds to and stabilize single stranded DNA. They keep the two strand separated by binding tightly to DNA. They provide template for synthesis of new DNA. Topoisomerase - Adds/subtracts supercoils and can remove the knots or loops in DNA. Supercoils are formed during replication. This problem can be overcome by group of enzymes called topoisomerases. Tus - binds to specific sequence of DNA opposite the ori and inhibits helicase. It acts as a counter helicase. It helps in ending DNA replication in prokaryotes.