2b) Suppose you generated a mutant E. coli strain in which it was DNA Polymerase I that was inactivated - assuming that all the other enzymes involved in replication remained fully functional, how would DNA replication in these mutant cells lacking DNA Pol I differ from DNA replication in normal E. coli? Briefly explain why you would expect to see that change/those changes in DNA replication in the mutant cells.

Q: Please explain one type of “DNA repair” mechanism (what is it, when is it used, how does it work).

A: The process by which a cell detects and repairs damage to the DNA molecules that encode its genome…

Q: This is a three part question: Figure B in Box 4.1 illustrates the results of the Meselson-Stahl…

A: The given figure shows the bands and graphical results after the two rounds of replication in…

Q: A pyrimidine dimer which is a bulky lesion has mutated an E. coli cell's DNA. Describe both the…

A: The DNA damage can be repaired by several mechanisms that includes direct repair, excision repair,…

Q: List the steps in the correct order of DNA mismatch repair in E. coli

A: The DNA mismatch repair process involves the correction of errors in the newly synthesized DNA. The…

Q: A mixture of four a-[32P]–labeled ribonucleoside triphosphates was added to permeabilized bacterial…

A: DNA replication is the process by which DNA makes a copy of itself during cell division. It is the…

Q: One way that bacterial cells regulate DNA replication is through GATC methylation sites within the…

A: The addition of the methyl group to the adenine of the newly synthesized strand of DNA precisely at…

Q: Ultraviolet light can cause covalent linkages between consecutive pyrimidine bases in DNA (up to 100…

A: The question is asking about the DNA repair system that is most effective in correcting the errors…

Q: The general excision repair pathway for DNA repair has the following order   A. endonuclease cuts…

A: When there is an exposure of any mutagens either UV radiation or chemicals, there is the occurrence…

Q: Question 5: (a) Would the addition of the molecule Actinomycin D affect the production of new HIV…

A: HIV(Human Immunodeficiency Virus) is the most dangerous virus that can affect the body by causing…

Q: Norflaxacin is a cidal broad spectrum antibiotic that functions by inhibiting DNA gyrase in gram +/-…

A: Norfloxacin is a broad-spectrum fluoroquinolone antibiotic, which is used to treat UTI (urinary…

Q: ndicate whether each of the following statements are true of depurination, deamination or pyrimidine…

A: DNA is a long polymer of nucleotide. A nucleotide is made ip of sugar, nitrogenous bases, and…

Q: 2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA Polymerase III, that…

A: Introduction DNA is the molecular structure that is composed of a pair of polynucleotide chains…

Q: Many of the gene products involved in DNA synthesis were initially defined by studying mutant E.…

A: During DNA replication another copy of DNA is synthesized on DNA template strand using Polymerase…

Q: Replication is occurring normally in these cells; would you expect to find a primer in both…

A: DNA replication is a semiconservative, semicontinuous, and bidirectional process. It occurs in the S…

Q: During cellular checkpoints DNA Repair Proteins can edit and patch our DNA through many different…

A: Cell cycle is broadly categorized into 2 phases such as interphase and M phase. Interphase consists…

Q: All known DNA polymerases catalyze synthesis only in the 5' → 3' direction.  Nevertheless, during…

A: DNA replication is the process of making two identical copies of DNA. The process is carried by the…

Q: Point mutations in multiple tumor suppressor proteins have been linked to cancer. For example…

A: In transcription only a segment of DNA and only one of the strand is copied into RNA.

Q: Please help with 2a) 2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA…

A: Replication is the process of duplication of two strands of a double stranded DNA. In bacteria, the…

Q: ) A bacterial chromosome contains 6.4 million nucleotides of DNA. If synthesis at each replication…

A: 1. It is given that a bacterial chromosome contains 6.4 million nucleotides of DNA. Suppose…

Q: Human Fbh1 helicase is important in the process of DNA replication. When a mutation occurs during…

A: Answer. DNA replication is a complex process involving numerous enzymes and proteins at the…

Q: 5) Both DNA polymerase (any DNA polymerase) and ligase catalyze the formation of a bond between…

A: DNA Polymerase and Ligase are the two classes of enzymes that take part in DNA replication and…

Tissue regeneration & repair

Tissue regeneration involves the restoration of tissue components by regrowth of damaged tissues. In contrast, tissue repair involves patching of injured tissue rather than restoring it. It is the proliferative capacity of the cells that decides the amount of regeneration against the repair that would occur in the tissue. Both regeneration and repair depending on the duration of the injury and response to the inflammation.