In a bacterial culture in which all cells are unable to synthesizeleucine (leu-), a potent mutagen is added, and the cells areallowed to undergo one round of replication. At that point, samplesare taken, a series of dilutions are made, and the cells areplated on either minimal medium or minimal medium containingleucine. The first culture condition (minimal medium) allowsthe growth of only leu+ cells, while the second culture condition(minimal medium with leucine added) allows growth of all cells.The results of the experiment are as follows: Culture Condition Dilution ColoniesMinimal medium 10-1 18Minimal medium + leucine 10-7 6What is the rate of mutation at the locus associated with leucinebiosynthesis?
In a bacterial culture in which all cells are unable to synthesize
leucine (leu-), a potent mutagen is added, and the cells are
allowed to undergo one round of replication. At that point, samples
are taken, a series of dilutions are made, and the cells are
plated on either minimal medium or minimal medium containing
leucine. The first culture condition (minimal medium) allows
the growth of only leu+ cells, while the second culture condition
(minimal medium with leucine added) allows growth of all cells.
The results of the experiment are as follows:
Culture Condition Dilution Colonies
Minimal medium 10-1 18
Minimal medium + leucine 10-7 6
What is the rate of mutation at the locus associated with leucine
biosynthesis?
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