Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which also served as a model system for the study of bacteriophage morphogenesis, DNA replication, and gene regulation. a) With the aid of a diagram, generally narrate how a foreign gene can be inserted into a lambda insertion vector and subsequently infect an Escherichia coli cell. b) You are cloning a 7.5 kb gene into a lambda gt10 vector, utilizing a restriction site which specifically present in the vector. State the restriction site that you can use for this purpose and suggest a screening procedure to indicate successful integration of the gene into the host genome.

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Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.