Answered: Bacteriophage lambda is as one of the…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which also
served as a model system for the study of bacteriophage morphogenesis, DNA replication, and
gene regulation.
a) With the aid of a diagram, generally narrate how a foreign gene can be inserted into a
lambda insertion vector and subsequently infect an Escherichia coli cell.
b) You are cloning a 7.5 kb gene into a lambda gt10 vector, utilizing a restriction site which
specifically present in the vector. State the restriction site that you can use for this purpose
and suggest a screening procedure to indicate successful integration of the gene into the
host genome.

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