Q6) Why are ‘sticky ends’ useful biotechnologically? Q7) Why is this enzyme called a restriction endonuclease? How is it different from an exonuclease?
Q6) Why are ‘sticky ends’ useful biotechnologically? Q7) Why is this enzyme called a restriction endonuclease? How is it different from an exonuclease?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Q6) Why are ‘sticky ends’ useful
Q7) Why is this enzyme called a restriction endonuclease? How is it different from an exonuclease?
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- When restriction enzymes cut between two bases on the same strand at one end of the sequence and two bases on the opposite end of the complimentary strand, a sticky end is formed.
- Sticky ends are more beneficial in molecular cloning, a typical biotechnology procedure, because they allow the DNA fragment to be put in the plasmid in the correct direction and the ligation of sticky ends requires less DNA than blunt ends due to their attraction to complimentary strands.
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