During your experiment you analysed only a few of the recombinant clones for the presence of the highly repeated Aluelements. If you wanted to screen for a single-copy gene, you would need to screen a much larger genomic library. Assuming, that you already know the amino acid sequence of unicorn (a species with a similar physiology to humans) insulin, how would you construct a probe which would enable you to use nucleic acid hybridisation to screen a unicorn genomic DNA library for the insulin gene? Hint: you have access to any molecular biology reagents and equipment you might need, such as vectors, enzymes, and DNA sequencers.

Q: You have isolated a cDNA clone encoding a protein of interest in a higher eukaryote. This cDNA clone…

A: cDNA  It is known as complementary DNA and made up of reverse transcription of mRNA.

Q: Could you explain how you would know if you successfully induced transformation? Thank you Did you…

A: Transformation experiments are very crucial for understanding fundamental biological processes.…

Q: During your experiment you analysed only a few of the recombinant clones for the presence of…

A: The objective of the question is to understand how to construct a probe for nucleic acid…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: Mutation analysis of GCK gene in patients with diabetes revealed a c.114 T→A (shown in bold and…

A: Restriction enzymes binds to the recognition sequence of the DNA and cuts the DNA into fragments.

Q: Propose a method for isolating a DNA fragment that is adjacent in the genome to a previously…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: The sequence at one end of one strand of the Drosophilatransposon Mariner is shown below (dots…

A: The genes are the sequence of nucleotides that are present on the chromosomes and encode for a…

Q: You are investigating the activity of different cadherins in cells. You use recombinant DNA…

A: The proteins in transmembrane that is useful for cell-cell adhesion. It plays a main role in…

Q: A new technique for rapidly determining the nuclesome structure for regions of chromatin is called…

A: In the field of molecular biology, understanding the mechanisms underlying gene expression…

Q: Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in…

A: CRISPR-Cas9 is a genome editing tool. It helps researchers to edit or change the gene from the…

Q: Draw roughly the comparative electrophoretic mobilities of close circular DNA, open circular DNA and…

A: Since there are multiple question asked in this particular question, I will answer the first one for…

Q: The soil bacterium Agrobacterium tumefaciens, also regarded as 'nature's own genetic engineer', is…

A: As per our guidelines, we are supposed to answer only three sub-parts. Kindly repost the question…

Q: Explain the difference in results for the RT-PCR from the CD4+ T cells and the RT-PCR from the CD8+…

A: RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a molecular technique commonly used to…

Q: A second enzyme has 4 differer fragments. If both enzymes are you took all the fragments from…

A: Restriction enzyme is a protein produced by bacteria that cleaves DNA at specific sites along the…

Q: If the malate-aspartate shuttle is inhibited under aerobic conditions, then lactate production…

A: Energy must be supplied to the cell continuously so that the cell maintains the functions and…

Q: Mutation analysis of GCK gene in patients with diabetes revealed a c.114 TA (shown in bold and…

A: Restriction enzyme are the enzyme which cuts the DNA at specific recognition site.

Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…

A: A promoter can be defined as a DNA sequence where proteins bind and initiate transcription of an…

Q: STEP BY STEP Exaplanation would be appreciated so I can understand for upcomming exam. In humans,…

A: Given that  GG =570 GT = 297 TT =33  Total population = 894

Q: c) Describe how plasmid PBR322 is used as a cloning vector. What are their special features? d) What…

A: Bacteria have extra chromosome apart from genomic chromosome . This extra chromosomal part have…

Q: Experiment: Transformation of the bacterium Streptococcus pneumoniae. Based on the study, four…

A: Introduction The genetic modification of a cell caused by the direct uptake and integration of…

Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: Avery, McLeod and McCarty experiment was used to determine the genetic material and its nature on an…

Q: Superpleural membrane protects the and resists the changes underlying ----- -- in interthoracic…

A: Answer: Human body is comprising a complex of various systems such as respiratory system ,…

Q: Besides cloning vectors, there are also expression vectors. What is the main differenc between these…

A: cloning vector is a small piece of DNA that can be inserted into organism for cloning purpose.…

Q: Shown below is an R loop prepared for electron microscopy by annealing a purified eukaryotic…

A: An exon is any part of a gene that will encode a part of the final mature RNA produced by that gene…

Q: You ran the agarose gel but forgot to include the lactose-induced 23S reaction (template - cDNA).…

A: If there were white colonies in the plate, then the recombinant  clone was created if blue then it…

Q: ingle stranded adaptor" and "homopo. " have different strategies of binding d

A: Cloning is a process that allows scientists to create perfect genetic duplicates of living…

Q: Although a large number of mutagenic chemicals are known,none is known that induces mutations in…

A: Mutation is the process of abrupt changes in the nucleotide sequence or gene in the DNA. Mutation…

Q: Expressing genes encoding a particular protein’s subunits from a plasmid in E. coli cells (volume of…

A: Escherichia coli (E. coli) are group of bacteria that are prevalent in the environment, foods, and…

Q: A site-directed mutagenesis experiment was done on the catalytic triad of the serine protease…

A: Mutagenesis is that the method through that mutations in DNA molecules arise. Mutations in DNA will…

Q: can you explain why we are hoping to obtain white colonies? (Please explain both why blue colonies…

A: In recombinant DNA technology vector is any DNA molecule either from plant, virus, plasmid or…

Q: Polymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983. This techniqu had indeed…

A: PCR is used to amplify a specific DNA sample.

Q: Mutation analysis of GCK gene in patients with diabetes revealed a c.114 T->A (shown in bold and…

A: Mutation analysis of GCK gene in patients with diabetes revealed a c.114 T ---->A (shown in bold…

Q: You know from your calculationsthat only a small proportion of the human genome is represented, even…

A: The objective of the question is to outline a strategy for constructing a genomic DNA library that…

Q: A number of yeast-derived elements were added to thecircular bacterial plasmid pBR322. Yeast that…

A: Genomics contains an important technique by which multiple and exact copies of a gene can be…

Q: Describe the rationale behind the electrophoretic mobility shift assay.

A: Nucleic acid is serving as primary information carrying molecule in cells. There are two types of…

Q: In the transformation experiment by Griffith, two strains of an organism was injected into a mouse…

A: Frederick Griffith was the man who performed the transformation principle on Streptococcus…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.