After Drosophila DNA has been treated with a restrictionenzyme, the fragments are inserted into plasmids and selected as clones in E. coli. With the use of this “shotgun”technique, every DNA sequence of Drosophila in a librarycan be recovered.a. How would you identify a clone that contains DNAencoding the protein actin, whose amino acid sequenceis known?b. How would you identify a clone encoding a specifictRNA

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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After Drosophila DNA has been treated with a restriction
enzyme, the fragments are inserted into plasmids and selected as clones in E. coli. With the use of this “shotgun”
technique, every DNA sequence of Drosophila in a library
can be recovered.
a. How would you identify a clone that contains DNA
encoding the protein actin, whose amino acid sequence
is known?
b. How would you identify a clone encoding a specific
tRNA

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