Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
a. Why doesn't Dpnl digest the mutagenized DNA?
b.What is the approximate size, in base pairs, of the pQE.1-CRYGD plasmid?
a. Why doesn't Dpnl digest the mutagenized DNA?
b.What is the approximate size, in base pairs, of the pQE.1-CRYGD plasmid?
Dpnl:- it is a restriction enzyme. It's genes are usually found in the plasmid of Escherichia coli.
Mutagenized DNA:- DNA having mutation is usually called as Mutagenized DNA. Mutation in DNA changes the DNA sequences.
Plasmid:- it is an extra chromosomal DNA. And usually found in bacteria.
Base pairs:- it is usually found in DNA as well as in RNA. Base pairs are formed when bases i.e. adenine, guanine, cytosine, and uracil (thymine is present at the place of uracil in DNA) binds with their complimentary bases. For example - Adenine with thymine and guanine with cytosine.
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