61. Which pairs of restriction enzymes would allow the cloning of the HGH cDNA 3' (downstream) of the TAP promoter in the plasmid shown to allow its expression in bacteria? a) Xbal BamH1 b) Xbal Kpnl c) R1 Sstl d) BamH1 Kpnl e) R1 Kpnl 5' « 4. RI ATG Xbal «< Direction of Transcription BamH1 Ssti Xbal BamH1 TAP Promoter TAA Sstl Kpn1 Kpnl HGH cDNA 3' Plasmid

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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61. Which pairs of restriction enzymes would allow the cloning of the HGH cDNA 3' (downstream) of the
TAP promoter in the plasmid
shown to allow its expression in
bacteria?
a) Xbal BamH1
b) Xbal Kpnl
c) R1 Sstl
d) BamH1 Kpnl
e) R1 Kpnl
4.
Ri
ATG
Xbal
«
Direction of
Transcription
BamHi
Ssil bai
BamH1
TAP Promoter
TAA
« ↑
Sst1 Kpn1
Kpn1
HGH cDNA
3¹
Plasmid
Transcribed Image Text:61. Which pairs of restriction enzymes would allow the cloning of the HGH cDNA 3' (downstream) of the TAP promoter in the plasmid shown to allow its expression in bacteria? a) Xbal BamH1 b) Xbal Kpnl c) R1 Sstl d) BamH1 Kpnl e) R1 Kpnl 4. Ri ATG Xbal « Direction of Transcription BamHi Ssil bai BamH1 TAP Promoter TAA « ↑ Sst1 Kpn1 Kpn1 HGH cDNA 3¹ Plasmid
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