61. Which pairs of restriction enzymes would allow the cloning of the HGH cDNA 3' (downstream) of the TAP promoter in the plasmid shown to allow its expression in bacteria? a) Xbal BamH1 b) Xbal Kpnl c) R1 Sstl d) BamH1 Kpnl e) R1 Kpnl 5' « 4. RI ATG Xbal «< Direction of Transcription BamH1 Ssti Xbal BamH1 TAP Promoter TAA Sstl Kpn1 Kpnl HGH cDNA 3' Plasmid
61. Which pairs of restriction enzymes would allow the cloning of the HGH cDNA 3' (downstream) of the TAP promoter in the plasmid shown to allow its expression in bacteria? a) Xbal BamH1 b) Xbal Kpnl c) R1 Sstl d) BamH1 Kpnl e) R1 Kpnl 5' « 4. RI ATG Xbal «< Direction of Transcription BamH1 Ssti Xbal BamH1 TAP Promoter TAA Sstl Kpn1 Kpnl HGH cDNA 3' Plasmid
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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