Answered: If you wanted to express a library of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

If you wanted to express a library of human proteins in yeast, there are several good reasons why it would be better to use a cDNA library instead of a genomic library from humans. Which one of the following options is not such a reason?

a. yeast may not initiate transcription or splicing of a human gene at the correct locations

b. some genes will have very high representation (many plasmids contain the gene), while others will have very low representation (few or no plasmids contain the gene) in the cDNA library

c. most genomic library clones would be useless, because only ~1.5% of the human genome actually encodes proteins

d. a cDNA library can contain multiple splice variants, which are common for human genes

e. introns are often very large in the human genome, making it impossible in many cases to contain a genomic version of an entire gene in a single plasmid

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