A doctoral student in biology, Esther Mensah, is reviewing primary literature over RNAi interference as part of her literary research for her proposal on decreasing the amount of abnormal white blood cell growth associated with acute myeloid leukemia (AML) using a murine (mouse) model. She is being provided with 10 AML mice courtesy of her mentor. Can you help her out with her proposal? 1) Esther has located the coding strand of cDNA from the AML gene that she wants to target for her RNA interference: ATG CCC GGT AAT CGC TTA TGC CTC. What will be the sequence of her siRNA? a AUG CCC GGU AAU CGC UUA UGC CUC. b TAC GGG CCA TTA GCG AAT ACG GAG. c CTC CGT ATU CGC TAA GGU CCC GUA. d UAC GGG CCA UUA GCG AAU ACG GAG. 2) RNAi works by inteferring with mRNA. Esther decides to design her RNAi strand using genomic DNA (gDNA) information. What is your opinion on her approach? a Smart move! You got this, girl! b It would be better if she designs the RNAi to bind to Drosha, since it is meant to bind to the transcript in the nuclease to avoid it from being transported to the cytoplasm where the ribosomes are. c She needs to work with gRNA not gDNA. d She needs to work with cDNA not gDNA since mRNA lacks introns unlike gDNA; this is necessary since it is meant to interfere with mature, spliced mRNA.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy

Section: Chapter Questions

Problem 13QP: The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future....

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A doctoral student in biology, Esther Mensah, is reviewing primary literature over RNAi interference as part of her literary research for her proposal on decreasing the amount of abnormal white blood cell growth associated with acute myeloid leukemia (AML) using a murine (mouse) model. She is being provided with 10 AML mice courtesy of her mentor. Can you help her out with her proposal?

Esther has located the coding strand of cDNA from the AML gene that she wants to target for her RNA interference: ATG CCC GGT AAT CGC TTA TGC CTC. What will be the sequence of her siRNA?

AUG CCC GGU AAU CGC UUA UGC CUC.

TAC GGG CCA TTA GCG AAT ACG GAG.

CTC CGT ATU CGC TAA GGU CCC GUA.

UAC GGG CCA UUA GCG AAU ACG GAG.

RNAi works by inteferring with mRNA. Esther decides to design her RNAi strand using genomic DNA (gDNA) information. What is your opinion on her approach?

Smart move! You got this, girl!

It would be better if she designs the RNAi to bind to Drosha, since it is meant to bind to the transcript in the nuclease to avoid it from being transported to the cytoplasm where the ribosomes are.

She needs to work with gRNA not gDNA.

She needs to work with cDNA not gDNA since mRNA lacks introns unlike gDNA; this is necessary since it is meant to interfere with mature, spliced mRNA.

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