Homework Help is Here – Start Your Trial Now!
Science
Biology
Refer to the experimental approach (i.e., very briefly describe) and give the results AND conclusions. The answer is incomplete without reference to all the data including controls. (A) To investigate whether the microRNA-145 (miR-145) plays a role in gastric cancer, levels of Ets1 and miR-145 in cancer and normal adjacent tissue of 84 patients were determined by qPCR. (B) Levels in normal (GES-1) and cancer cell lines were determined. (C) Cancer cell lines were transfected with the Ets1 3'-UTR reporter plasmid and either miR-145 or negative control (mock), and luciferase assays were carried out. (D) Western blots of cells transfected with miR-145 or mock stained for Etsl

Refer to the experimental approach (i.e., very briefly describe) and give the results AND conclusions. The answer is incomplete without reference to all the data including controls. (A) To investigate whether the microRNA-145 (miR-145) plays a role in gastric cancer, levels of Ets1 and miR-145 in cancer and normal adjacent tissue of 84 patients were determined by qPCR. (B) Levels in normal (GES-1) and cancer cell lines were determined. (C) Cancer cell lines were transfected with the Ets1 3'-UTR reporter plasmid and either miR-145 or negative control (mock), and luciferase assays were carried out. (D) Western blots of cells transfected with miR-145 or mock stained for Etsl

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
100%

Please help me with the Cellular Biology question below. It is one question, the data given is to help answer it. 

Please be as detailed as possible.

**Investigation of miR-145 Role in Gastric Cancer** **Experimental Approach:** 1. **Objective:** - To examine whether microRNA-145 (miR-145) influences gastric cancer by comparing levels of Ets1 and miR-145 in both cancerous and normal adjacent tissues from 84 patients using qPCR. 2. **Specific Steps:** - **(A)** Measure the levels of miR-145 and Ets1 in cancerous and corresponding normal tissues. - **(B)** Compare these levels in normal (GES-1) vs. cancer cell lines. - **(C)** Transfect cancer cell lines with Ets1 3’-UTR reporter plasmid and either miR-145 or a control, and then perform luciferase assays. - **(D)** Perform Western blots for cells transfected with miR-145 or mock to detect Ets1 presence. **Results:** **A. Relative Levels of Ets1 and miR-145 in Tissues (Figure A):** 1. **Ets1 Expression:** - The box plot on the left shows a significant increase in Ets1 transcript levels in cancer tissues compared to normal adjacent tissues. - p < 0.0001 indicates a highly significant difference. 2. **miR-145 Expression:** - The box plot on the right demonstrates a significant decrease in miR-145 expression in cancer tissues compared to normal tissues. - p < 0.0001 also indicates a highly significant difference. **B. Relative Transcript Levels in Cell Lines (Figure B):** - Bar graph presenting Ets1 and miR-145 expression levels across different cell lines (GES-1, AGS, SGC-7901, MKN-45). - miR-145 and Ets1 expression levels vary significantly with lower miR-145 and higher Ets1 in cancer cell lines compared to GES-1 cells. - '*' indicates statistical significance. **C. Luciferase Assay with Ets1 3’-UTR Reporter Plasmid (Figure C):** - Schematic diagram outlining the constructs used for the luciferase assay with wild-type (WT) and mutant (Mut) Ets1 3’-UTR sequences. - The sequences provided for both WT and Mut Ets1 3’-UTR
Transcribed Image Text:**Investigation of miR-145 Role in Gastric Cancer** **Experimental Approach:** 1. **Objective:** - To examine whether microRNA-145 (miR-145) influences gastric cancer by comparing levels of Ets1 and miR-145 in both cancerous and normal adjacent tissues from 84 patients using qPCR. 2. **Specific Steps:** - **(A)** Measure the levels of miR-145 and Ets1 in cancerous and corresponding normal tissues. - **(B)** Compare these levels in normal (GES-1) vs. cancer cell lines. - **(C)** Transfect cancer cell lines with Ets1 3’-UTR reporter plasmid and either miR-145 or a control, and then perform luciferase assays. - **(D)** Perform Western blots for cells transfected with miR-145 or mock to detect Ets1 presence. **Results:** **A. Relative Levels of Ets1 and miR-145 in Tissues (Figure A):** 1. **Ets1 Expression:** - The box plot on the left shows a significant increase in Ets1 transcript levels in cancer tissues compared to normal adjacent tissues. - p < 0.0001 indicates a highly significant difference. 2. **miR-145 Expression:** - The box plot on the right demonstrates a significant decrease in miR-145 expression in cancer tissues compared to normal tissues. - p < 0.0001 also indicates a highly significant difference. **B. Relative Transcript Levels in Cell Lines (Figure B):** - Bar graph presenting Ets1 and miR-145 expression levels across different cell lines (GES-1, AGS, SGC-7901, MKN-45). - miR-145 and Ets1 expression levels vary significantly with lower miR-145 and higher Ets1 in cancer cell lines compared to GES-1 cells. - '*' indicates statistical significance. **C. Luciferase Assay with Ets1 3’-UTR Reporter Plasmid (Figure C):** - Schematic diagram outlining the constructs used for the luciferase assay with wild-type (WT) and mutant (Mut) Ets1 3’-UTR sequences. - The sequences provided for both WT and Mut Ets1 3’-UTR
Expert Solution
steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genomic studies
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS