You are working in the lab and are mutagenizing E. coli, to see if you can identify mutations in the DNA that affect the function of E. coli. Instead of trying to grow the mutants and look for phenotypic defects, you decide to sequence the genome of a few of your mutants. One of the mutant E. coli has a mutation in the gene for tRNA nucleotidyltransferase. Now you decide to try to grow this E. coli mutant in the lab, but you are unable to detect any expansion of the population of bacterial cells. What is a possible mechanistic explanation for the lack of population growth of this mutant E. coli?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
You are working in the lab and are mutagenizing E. coli, to see if you can identify mutations in the DNA that affect the function of E. coli. Instead of trying to grow the mutants and look for
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