You are working in a research lab that is surveying the CFTR gene sequence in humans to identify new alleles of the CFTR gene. Shown below is a portion of the CFTR protein-coding sequence of the gene from a person who does not have cystic fibrosis, as well as the mRNA that is produced from transcription from this gene. You can assume that the first codon starts right at 5’ end of the mRNA (we have included spacing between the codons to make it easier to translate). Coding DNA 5’- ATG GTG ACT CGT TGT GCC ATT GCA GCT AAA CAC TTC GAG CCC TGT- 3’ Template. 3’- TAC CAC TGA GCA ACA CGG TAA CGT CGA TTT GTG AAG CTC GGG ACA- 5’ mRNA 5’- AUG GUG ACU CGU UGU GCC AUU GCA GCU AAA CAC UUC GAG CCC UGU- 3’ I. What is the polypeptide sequence? You have discovered two NEW alleles. The DNA and mRNA sequences for the two new alleles that you have identified are shown below (mutation in DNA is underlined and bolded in black). II. For alleles X and Y, please: 1) write out the polypeptide sequence; and 2) Identify the type of mutation in allele X and allele W (missense, nonsense or silent mutation). Allele X Coding DNA 5’- ATG GTG ACT CGT TGT GCC ATT GCA GCT AAA CAT TTC GAG CCC TGT- 3’ Template 3’- TAC CAC TGA GCA ACA CGG TAA CGT CGA TTT GTG AAG CTC GGG ACA- 5’ mRNA 5’- AUG GUG ACU CGU UGU GCC AUU GCA GCU AAA CAU UUC GAG CCC UGU- 3’ Polypeptide Sequence: Mutation Type: Allele W Coding DNA 5’- ATG GTG ACT CGT TGA GCC ATT GCA GCT AAA CAC TTC GAG CCC TGT- 3’ Template. 3’- TAC CAC TGA GCA ACA CGG TAA CGT CGA TTT GTG AAG CTC GGG ACA- 5’ mRNA. 5’- AUG GUG ACU CGU UGA GCC AUU GCA GCU AAA CAC UUC GAG CCC UGU- 3’ Polypeptide Sequence: Mutation Type:
You are working in a research lab that is surveying the CFTR gene sequence in humans to identify
new alleles of the CFTR gene. Shown below is a portion of the CFTR protein-coding sequence of the
gene from a person who does not have cystic fibrosis, as well as the mRNA that is produced from
transcription from this gene. You can assume that the first codon starts right at 5’ end of the mRNA
(we have included spacing between the codons to make it easier to translate).
Coding DNA 5’- ATG GTG ACT CGT TGT GCC ATT GCA GCT AAA CAC TTC GAG CCC TGT- 3’
Template. 3’- TAC CAC TGA GCA ACA CGG TAA CGT CGA TTT GTG AAG CTC GGG ACA- 5’
mRNA 5’- AUG GUG ACU CGU UGU GCC AUU GCA GCU AAA CAC UUC GAG CCC UGU- 3’
I. What is the polypeptide sequence?
You have discovered two NEW alleles. The DNA and mRNA sequences for the two new alleles that
you have identified are shown below (mutation in DNA is underlined and bolded in black).
II. For alleles X and Y, please: 1) write out the polypeptide sequence; and 2) Identify the type of
mutation in allele X and allele W (missense, nonsense or silent mutation).
Allele X
Coding DNA 5’- ATG GTG ACT CGT TGT GCC ATT GCA GCT AAA CAT TTC GAG CCC TGT- 3’
Template 3’- TAC CAC TGA GCA ACA CGG TAA CGT CGA TTT GTG AAG CTC GGG ACA- 5’
mRNA 5’- AUG GUG ACU CGU UGU GCC AUU GCA GCU AAA CAU UUC GAG CCC UGU- 3’
Polypeptide
Sequence:
Mutation Type:
Allele W
Coding DNA 5’- ATG GTG ACT CGT TGA GCC ATT GCA GCT AAA CAC TTC GAG CCC TGT- 3’
Template. 3’- TAC CAC TGA GCA ACA CGG TAA CGT CGA TTT GTG AAG CTC GGG ACA- 5’
mRNA. 5’- AUG GUG ACU CGU UGA GCC AUU GCA GCU AAA CAC UUC GAG CCC UGU- 3’
Polypeptide
Sequence:
Mutation Type:
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