Imagine you are going to label a gene associated with apoptosis in Symbiodiniaceae with a Yellow Fluorescent Protein (YFP). To generate the YFP, you know the pre-mRNA looks as follows: Unspliced YFP premature mRNA Cap 5' UTR Exon 1 Intron Exon 2 Intron Exon 3 3' UTR If Exon 2 is also required for mRNA stability, what can be predicted from the possible spliced alternative isoforms formed? One of the isoforms will not have a poly-A tail The alternative splicing of YFP pre-mRNA prevents 5'-capping The mRNA isoform without Exon 2 will be degraded faster than the other isoform Exon 2 will be added to isoform B later to correct the mistake in splicing The protein translated from one of the mRNA isoforms will possess an additional functional domain Poly-A tail
Imagine you are going to label a gene associated with apoptosis in Symbiodiniaceae with a Yellow Fluorescent Protein (YFP). To generate the YFP, you know the pre-mRNA looks as follows: Unspliced YFP premature mRNA Cap 5' UTR Exon 1 Intron Exon 2 Intron Exon 3 3' UTR If Exon 2 is also required for mRNA stability, what can be predicted from the possible spliced alternative isoforms formed? One of the isoforms will not have a poly-A tail The alternative splicing of YFP pre-mRNA prevents 5'-capping The mRNA isoform without Exon 2 will be degraded faster than the other isoform Exon 2 will be added to isoform B later to correct the mistake in splicing The protein translated from one of the mRNA isoforms will possess an additional functional domain Poly-A tail
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Imagine you are going to label a gene associated with apoptosis in
Symbiodiniaceae with a Yellow Fluorescent Protein (YFP). To
generate the YFP, you know the pre-MRNA looks as follows:
Unspliced YFP premature mRNA
Сap
5' UTR
Exon 1
Intron
Exon 2
Intron
Exon 3
3' UTR
Poly-A tail
If Exon 2 is also required for mRNA stability, what can be predicted
from the possible spliced alternative isoforms formed?
One of the isoforms will not have a poly-A tail
O The alternative splicing of YFP pre-MRNA prevents 5'-capping
The MRNA isoform without Exon 2 will be degraded faster than the other
isoform
Exon 2 will be added to isoform B later to correct the mistake in splicing
The protein translated from one of the mRNA isoforms will possess an
additional functional domain
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