E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into the expression plasmid pET28a (Novagen), where expression is under control of the inducible T7 promoter. Give information about the enzyme being purified, its expression in E. coli and the property being used for its purification. please provide the references for the answer.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into the expression plasmid pET28a (Novagen), where expression is under control of the inducible T7 promoter.
Give information about the enzyme being purified, its expression in E. coli and the property being used for its purification. please provide the references for the answer.
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