You want to clone the gene Dik1 into a plasmid that will be used to generate a transgenic mouse. The goal is for DIk1 to be expressed exclusively in the muscle. "MCK prom" denotes a promoter from a gene expressed exclusively in muscle. KanR is Kanamycin resistance gene, which acts as a selectable marker in this plasmid. Based on the illustrations of the plasmid and the Dlk1 gene, which restriction enzyme(s) should you use to clone Dlk1 into this plasmid? Konl BamHI Sall Psti EcoRI Kpnl ATGACT TACTGA. .TACTGA ATGACT 5° Kpni Bam ORI MCK prom -EcoRI Diki ORF 1.5 kb 重一 BamHI, Pstl Sall, Pstl Kpnl, Pstl Kpnl BamHI, EcoRI

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