You want to clone the gene Dik1 into a plasmid that will be used to generate a transgenic mouse. The goal is for DIk1 to be expressed exclusively in the muscle. "MCK prom" denotes a promoter from a gene expressed exclusively in muscle. KanR is Kanamycin resistance gene, which acts as a selectable marker in this plasmid. Based on the illustrations of the plasmid and the Dlk1 gene, which restriction enzyme(s) should you use to clone Dlk1 into this plasmid? Konl BamHI Sall Psti EcoRI Kpnl ATGACT TACTGA. .TACTGA ATGACT 5° Kpni Bam ORI MCK prom -EcoRI Diki ORF 1.5 kb 重一 BamHI, Pstl Sall, Pstl Kpnl, Pstl Kpnl BamHI, EcoRI

You want to clone the gene Dik1 into a plasmid that will be used to generate a transgenic mouse. The goal is for DIk1 to be expressed exclusively in the muscle. "MCK prom" denotes a promoter from a gene expressed exclusively in muscle. KanR is Kanamycin resistance gene, which acts as a selectable marker in this plasmid. Based on the illustrations of the plasmid and the Dlk1 gene, which restriction enzyme(s) should you use to clone Dlk1 into this plasmid? Konl BamHI Sall Psti EcoRI Kpnl ATGACT TACTGA. .TACTGA ATGACT 5° Kpni Bam ORI MCK prom -EcoRI Diki ORF 1.5 kb 重一 BamHI, Pstl Sall, Pstl Kpnl, Pstl Kpnl BamHI, EcoRI

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?
Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.
The pre-mRNA transcript and protein made by several mutant genes were examined. The results are given below. Determine where in the gene a likely mutation lies: the promoter region, exon, intron, cap on mRNA, or ribosome binding site. a. normal-length transcript, normal-length nonfunctional protein b. normal-length transcript, no protein made c. normal-length transcript, normal-length mRNA, short nonfunctional protein d. normal-length transcript, longer mRNA, shorter nonfunctional protein e. transcript never made
You learned in the chapter that an STR locus is a locus where alleles differ in the number of copies of a short, tandemly repeated DNA sequence. PCR is used to determine the number of alleles present, as shown by the size of the DNA fragment amplified. In the Figure below are the results of PCR analysis for STR alleles at a locus where the repeat unit length is 9 bp, and alleles are known that have 5 to 11 copies of the repeat. Given the STR alleles present in the adults, state whether each of the four juveniles could or could not be an off-spring of those two adults. Explain your answers.
Match each term with the most suitable description. ___ DNA profile a. GMO with a foreign gene ___ Ti plasmid b. alleles commonly contain them ___ cDNA synthesis c. a persons unique collection of short tandem repeats ___ SNP d. requires reverse transcriptase ___ transgenic e. cuts DNA ___ restriction enzyme f. used in plant gene transfers
Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?
Given the following tRNA anticodon sequence, derive the mRNA and the DNA template strand. Also, write out the amino acid sequence of the protein encoded by this message. tRNA: UAC UCU CGA GGC mRNA: protein: How many hydrogen bonds would be present in the DNA segment?
The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future. Gene therapy for adenosine deaminase deficiency has proved to be quite promising, but many obstacles remain to be overcome. Currently, the correction of human genetic defects is done using retroviruses as vectors. For this purpose, viral genes are removed from the retroviral genome, creating a vector capable of transferring human structural genes into sites on human chromosomes within target-tissue cells. Do you see any potential problems with inserting pieces of a retroviral genome into humans? If so, are there ways to combat or prevent these problems?
An individuals set of unique _______ can be used as a DNA profile. a. DNA sequence c. short tandem repeats b. SNPs d. all of the above
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.