Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Textbook Question
Chapter 18, Problem 3TYK
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors?
a. The plasmid must have resistance to accept DNA inserts.
b. They allow the detection of plasmids that contain an inserted DNA fragment.
c. They ensure the presence of the ori site.
d. They ensure that the plasmid can be cut by a restriction enzyme.
e. They allow identification of bacteria that have taken up a plasmid.
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Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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- Plasmids are important for recombining DNA. B.) What are the requirments of an expression vector.arrow_forwardA plasmid has two restriction cut sites for EcoRI and one restriction cut site for HindIII. The plasmid is subjected to PCR, and the region containing the three restriction cut sites is amplified, such that all three sites are present in the resulting amplicon. If the amplicon is incubated with EcoRI, how many fragments of DNA are expected to result from this digest experiment? a. 0 b. 1 c. 2 d. 3 e. 4arrow_forwardPlasmids are very important for recombinant DNA work. 1.) Describe the components that are important to a cloning vector.arrow_forward
- A molecular geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. He knows that the nucleotide sequence of a small part of the gene is CTCGACTCACA. Briefly explain how to obtain the desired gene. Briefly describe how to clone the desired gene into a cloning plasmid.arrow_forwardExplain why the genes whose products are required for the normal growth of bacteria not carried on plasmids? Give two examples (one bacterial gene and one plasmid gene) to support your answer.arrow_forwardWhy do prokaryotes produce restriction enzymes? A. Restriction enzymes are produced to enable bacteria to repair lesions in its DNA B. Restriction enzymes enable prokaryotes to assimilate genes or fragments of DNA from its environment C. Restriction enzymes excise phage DNA from prokaryotes thereby protecting them from lysis. D. Restriction enzymes help unwind DNA and facilitate DNA replication in prokaryotes E. Restriction enzymes are necessary for editing DNA during replication.arrow_forward
- Which of the following one is liable for the conversion of covalently closed circular DNA to super coiled DNA of the plasmid? a.Exonuclease b. DNA Gyras c. Endonuclease d. Topoisomerasearrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forwardIf a F plasmid does not have the gene encoding TraJ, this F plasmid would Select one: a. become more active for conjugation O b. become more active for transformation. O c. become less active for transformation. O d. become less active for conjugationarrow_forward
- When plasmids are isolated from bacterial cells, they may existin a number of forms.a. List the different forms that may be found.b. Which do you think would migrate the fastest and farthest in anelectrophoresis experiment and why?arrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forwarda. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?arrow_forward
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