Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 18, Problem 10TYK
Summary Introduction
Introduction:
DNA fingerprinting is a method which is used to identify the DNA (deoxyribonucleic acid) of different species based on the occurrence of variable number tandem repeats (VNTR). It is done through restriction digestion of
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Which of the following is true about DNA manipulation? Select the best answer.
A. all of these
B. DNA has never been transferred between different organisms.
C. Transferring DNA from one organism into another always kills the host organism.
D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques.
E. DNA can't be manipulated.
Place the following stages of a physical mapping study in theirmost logical order:A. Clone large fragments of DNA to make a BAC library.B. Determine the DNA sequence of subclones from a cosmidlibrary.C. Subclone BAC fragments to make a cosmid library.D. Subclone cosmid fragments for DNA sequencing.
1
2
Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will
model the process yourself to solve a crime. Good luck, detective!
Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic
technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been
isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA
is shown in Figure 5.
10
50
DNA Profile from Forehead Print
Number of base pairs
00
50
40
D58818
075830
I
16
MU
DES1179
Shandand
(10)
70
CSF1PO
DITS820
80
100
Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the
core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179).
and data for the four STR loci that were included in the
A suspect was identified in the case. Her DNA…
Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Choose the one answer that fits best. Which of the following statements regarding DNA Fingerprinting is NOT correct (videos)? a. Fingerprinting is a technique that allows us to sequence an entire genome b. Forensic DNA profiling uses 13 different regions of DNA (according to the video) c. STRs allow us to tell individuals apart d. Short tandem repeats are in the non-coding regions e. DNA Fingerprinting is used in Forensic Sciencesarrow_forwardThe BLAST program is a tool fora. inserting many DNA fragments into a cell at the same time.b. translating a DNA sequence into an amino acid sequence.c. identifying homology between a selected sequence and geneticsequences in databases.d. all of the above.e. both b and c.arrow_forwardPCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller fragments. O b. Determine if a particular piece of DNA contains a certain gene. Oc. Amplify a specific segment of DNA. d. Separate DNA fragments based on size. e. Isolate specific pieces of DNA. ess Jump to...arrow_forward
- RELPs: A. are the same length for mutant and normal beta-globin alleles. b. determine the sequence of bases in a DNA fragment. c. have short, palindromic sequences of DNA in the middle. D. are used as vectors. e. are produced by reaction with restriction enzymes and are detected with PCR and gel electrophoresis.arrow_forwarda. It is possible to perform DNA fingerprinting withSNPs instead of SSRs as DNA markers, but ingeneral you would need to examine more SNPmarkers than the 13 SSRs used in the CODIS database to be sure of a match. Explain why.b. DNA fingerprinting has been used to verify pedigrees of valuable animals such as show dogs, racinggreyhounds, and thoroughbred horses. However, thetechnology is much harder to apply in these casesthan it is in forensic applications for humans. In particular, many more DNA markers must be examinedin domesticated animals to establish the identity orclose familial relationship of two DNA samples.Why would you need to look at more polymorphicloci in these animals than you would in humans?arrow_forwardWhich of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.arrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwarda. DNA fingerprinting is a method of visualizing similarities and differences in the DNA sequence of individuals. Arrange the following items so that they show the DNA fingerprinting steps in proper order: - Gel electrophoresis - Stain or probe for DNA - cut DNA into fragments - Amplify DNA sample using PCR b. On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?arrow_forwardThe rate of migration of DNA within an agarose gel in the gel electrophoresis technique is dependent on what factor(s)? Select one or more: a. G-C /A-T ratio b. Well size of the gel c. Size of DNA fragment d. Volume of sample loaded e. Negative charge of DNAarrow_forward
- Some restriction enzymes produce DNA fragments with overhanging stretches called sticky ends on each strand. Sticky ends are useful in making recombinant DNA because Select one: a. Sticky ends contain the exact same nucleotides that allows fragments to splice together. b. Sticky ends contain nucleotides with complementary bases that allows fragments to splice together. c. Sticky ends contain the exact same nucleotides that can form hydrogen bonds. d. Sticky ends contain nucleotides with complementary bases that can form hydrogen bonds.arrow_forwardComparing SNPs to STR markers, which of the following are true? Select one: a. SNP loci generally have more alleles than STR loci. b. SNP loci could not be used for forensic CODIS DNA profiling. c. SNPs are not the same as microsatellite DNA. d. There are many more STR loci in the human genome than SNP markers.arrow_forwardPage < Lab Procedure: The forensic specialist sends you the DNA from the crime scene (CS), and DNA from four different suspects (suspects A, B, C, D). You perform a PCR with each DNA sample. Using primers specific to the DNA sequences on either side of the STR, you amplify billions of copies of each of the two original TH01 alleles in each DNA sample (CS, A, B, C, and D). Then you run an agarose gel (DNA gel electrophoresis), which separates the DNA fragments by size. You obtained the following results (picture of DNA gel): CS A B C D Ladderarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Concepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax College
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Concepts of Biology
Biology
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:OpenStax College
Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License