Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 18, Problem 10TYK
Summary Introduction
Introduction:
DNA fingerprinting is a method which is used to identify the DNA (deoxyribonucleic acid) of different species based on the occurrence of variable number tandem repeats (VNTR). It is done through restriction digestion of
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Which of the following is true about DNA manipulation? Select the best answer.
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B. DNA has never been transferred between different organisms.
C. Transferring DNA from one organism into another always kills the host organism.
D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques.
E. DNA can't be manipulated.
Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?
a.
Chain termination occurs during synthesis of a new DNA strand.
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Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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- Place the following stages of a physical mapping study in theirmost logical order:A. Clone large fragments of DNA to make a BAC library.B. Determine the DNA sequence of subclones from a cosmidlibrary.C. Subclone BAC fragments to make a cosmid library.D. Subclone cosmid fragments for DNA sequencing.arrow_forwardWhat is the purpose of: A. The concentrated salt solution? B. adding isopropyl alcohol to the solution? Why does it need to be in low temperature?arrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forward
- The BLAST program is a tool fora. inserting many DNA fragments into a cell at the same time.b. translating a DNA sequence into an amino acid sequence.c. identifying homology between a selected sequence and geneticsequences in databases.d. all of the above.e. both b and c.arrow_forwardWhich statement is true? a. There is no danger involved in recombinant DNA research in humans. b. Stringent safety rules make the use of recombinant DNA research impossible. c. There is no danger in releasing recombinant organisms into the environment. d. Stringent safety rules make the use of recombinant DNA research possible. e. There is no danger involved in recombinant DNA research in bacteria.arrow_forwardBy mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look?A. The sequence will be readable for the first 20 bases only.B. The detector will detect two nucleotides at about 75% of the positions.C. The sequence will be readable for the last 20 bases only.D. The detector will detect one nucleotide at about 75% of the positions.E. The detector will detect no nucleotides at any of the positions. Explain why it is B.arrow_forward
- PCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller fragments. O b. Determine if a particular piece of DNA contains a certain gene. Oc. Amplify a specific segment of DNA. d. Separate DNA fragments based on size. e. Isolate specific pieces of DNA. ess Jump to...arrow_forwardMatch the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.arrow_forwardGel electrophoresis can be used to separate DNA on the basis of: A. the probe used. B. size. C. G and C nucleotide content. D. both size and loading buffer content. E. loading buffer content.arrow_forward
- Give detailed Solution with explanation neededarrow_forwarda. It is possible to perform DNA fingerprinting withSNPs instead of SSRs as DNA markers, but ingeneral you would need to examine more SNPmarkers than the 13 SSRs used in the CODIS database to be sure of a match. Explain why.b. DNA fingerprinting has been used to verify pedigrees of valuable animals such as show dogs, racinggreyhounds, and thoroughbred horses. However, thetechnology is much harder to apply in these casesthan it is in forensic applications for humans. In particular, many more DNA markers must be examinedin domesticated animals to establish the identity orclose familial relationship of two DNA samples.Why would you need to look at more polymorphicloci in these animals than you would in humans?arrow_forwardWhich of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.arrow_forward
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