Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 18, Problem 6TYK
Summary Introduction
Introduction:
Genetic engineering involves the production of a transgenic organism or a genetically modified organism (GMO). The bacteria in this process are engineered to carry a vector carrying a transgene of the desired character. The desired proteins expressed in the bacteria can be used in medical, industrial, and research industries.
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Students have asked these similar questions
A
has been assembled by researchers and transplanted into a donor bacterial strain to study never
before seen gene functions.
Select one:
a. Transgenic genome
b. Recombinant DNA sequence
c. Knockdown gene
d. Synthetic genome
o e. Recombinant plasmid
Clear my choice
is changing our
Sequencing the human genome, the development of microarray technology, and
understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic
disease by comparing the healthy tissue of individuals against the disease state of others.
Select one:
C a. Proteomics
o b. Metagenomics
MO C. Functional genomics
d. Personal genomics
O e. Developmental genomics
Clear my choice
Plasmids are important in biotechnology because they are
Group of answer choices
a. a vehicle for the insertion of foreign genes into bacteria
b. recognition sites on recombinant DNA strands
c. surfaces for protein synthesis in eukaryotic recombinants
d. viruses incorporated into the host DNA
Pair the appropriate item with its purpose in the production of insulin
a DNA containing the gene for insulin.
b Restriction enzymes and DNA ligase
c PBR322 (a plasmid)
d Lac promoter (regulatory sequence)
e IPTG
Triggers gene expression
Provides sequences needed for RNA polymerase to bind and
transcribe.
Provides a means of getting the DNA into the bacterium and
keeping it there.
Allows DNA to be cut and put back together in order to produce
the needed construct.
Contains the sequence the bacterium needs in order to produce
insulin.
Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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- Which of the following cannot usually be inserted into a vector to form a recombinant DNA? A. PCR product of a eukaryotic coding sequence B. Lac operon operator region DNA sequence C. Reverse transcriptase gene D. E. coliarrow_forwardDescribe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.arrow_forwardWhich of the following one is liable for the conversion of covalently closed circular DNA to super coiled DNA of the plasmid? a.Exonuclease b. DNA Gyras c. Endonuclease d. Topoisomerasearrow_forward
- If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know if the protein encoded by the cloned gene is expressed or not, which of the following methods is the right one to use? Select one: a. Northern blot b. Both Northern and Western blots c. Agarose gel with polyacrylamide d. Western blot e. Protein gel and northern blotarrow_forwardWhat is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blotarrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forward
- has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a Transgenic genome b. Recombinant DNA sequence C. Knockdown gene d. Synthetic genome e. Recombinant plasmid xarrow_forwardOne would be correct in assuming that the restriction sites present in the multiplecloning site of the pUC18 plasmid willarrow_forwardYou are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a test group of strawberry plants. Select the events you would use to genetically engineer these plants. Insert the ice gene into the T-DNA region of a Ti plasmid; transform Agrobacterium tumefaciens with this plasmid; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. Insert the ice gene into the T-DNA region of the Agrobacterium tumefaciens chromosome; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. O Insert the ice gene into a Ti transposon; Introduce the transposon into Agrobacterium tumefaciens Ti plasmid: Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens: Select plant cells that have taken up the ice gene. Insert the ice gene into a Ti bacteriophage; transduce Agrobacterium tumefaciens with this…arrow_forward
- A.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…arrow_forwardMatch items in Column A with most related ones in B. Write the letters on the space provided.arrow_forwardBacteria can be used to produce human growth hormone (HGH - a peptide/protein) through genetic engineering. The human gene for HGH is inserted into a plasmid, which is then taken up by a bacterial cell, which divides and multiplies into a clone of cells, all of which contain the plasmid with the HGH gene. The bacteria express the HGH gene, producing HGH which can be harvested and used for treatment of humans. (See figure below) Which of the following statements is NOT true about this process? bacterium Vector, such as a DNA containing the gene of plasmid, isolated it from a different species is Gene encoding protein for pest resistance is inserted into plant cells ©2019 Pearson Education, Inc chromosome recombinant DNA (plasmid) transformed bacterium Create and harvest copies of a gene with either of two goals in mind. Gene encoding degradative enzyme to clean up toxdo waste is inserted into bacterial cells ved by an enzyme into gene of interest The desired gene is selected and…arrow_forward
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