Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 18, Problem 2TYK
Summary Introduction
Introduction:
The recombinant DNA is made by using two or more DNA fragments of different species or organisms. The fragments cleave by some specific bacterial enzymes, which are known as restriction endonucleases or restriction enzymes. These fragments are joined by the enzymes known as ligases.
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Definition of Terms:
a. Genetic Engineering
b. DNA
c. Recombinant DNA
d. Plasmids
e. Cloning
f. Genome
g. Gene Mapping
h. Biotechnology
i. Polymerase Chain Reaction
j. Gene Therapy
For each situation, write the letter of the technique that would be most helpful;
A. DNA editing
A doctor wants to know if a patient has an inherited
using CRISPR
B. DNA replication
using PCR
C. DNA analysis
through genetic
testing
D. DNA insertion
16.
disorder.
I
A scientist needs many copies of a gene to conduct an
17.
experiment.
A genetic engineer wants to replace a defective copy of
a gene with a functional copy in a chromosome.
18.
into bacteria as a
plasmid
A medical researcher needs many copies of a protein
19.
(insulin) to be produced to use in a medical treatment.
A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one
produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter
one letter in each blanks (19 & 20) to correctly complete the sentences.
ninate Education TM, Inc.
A
has been assembled by researchers and transplanted into a donor bacterial strain to study never
before seen gene functions.
Select one:
a. Transgenic genome
b. Recombinant DNA sequence
c. Knockdown gene
d. Synthetic genome
o e. Recombinant plasmid
Clear my choice
is changing our
Sequencing the human genome, the development of microarray technology, and
understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic
disease by comparing the healthy tissue of individuals against the disease state of others.
Select one:
C a. Proteomics
o b. Metagenomics
MO C. Functional genomics
d. Personal genomics
O e. Developmental genomics
Clear my choice
Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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- The CRISPR method is used in a.DNA sequencing. b.DNA fingerprinting. c.genome editing. d.the polymerase chain reaction.arrow_forwardWhich of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forwardTransgenic bacteria can be used to make an alanine rich (GM) plant. Explain how bacteria can be used to produce large amounts as a cheap source of protein. Your explanation will include the role of: Restriction enzymes, plasmids, recombinant DNA, and bacteria.arrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardThe other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedarrow_forwardWhat would be the effect of performing a RT-PCR with the following ingredients: an mRNA template, appropriate primers, dNTPs, heat-stable reverse transcriptase and human DNA polymerase? Select one: a. The PCR would occur, but with a high mutation rate b. The PCR reaction will not commence c. Non-specific PCR of random templates will occur d. The RNA template would be converted to DNA, but the DNA segment would not be amplified PCR would proceed normally е.arrow_forward
- Which of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.arrow_forwardMatch the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.arrow_forwardCan you help me with this question, please? What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forward
- CHOICES: a. Plasmid b. Sticky end c. DNA ligase d. Transformation e. Restriction enzyme f. Genetic marker g. Transduction QUESTIONS: Cuts the DNA into fragments Circular DNA molecule of bacteria Used to insert DNA of interest to vector Area of DNA where bases are ready to be paired Recombinant DNA technology with the help of a vector genearrow_forwardWhich of the following best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers hybridize to the target DNA. II-The mixture is heated to a high temperature to denature the double stranded target DNA. III-Fresh DNA polymerase is added. IV-DNA polymerase extends the primers to make a copy of the target DNA. A) II, I, IV. B) I, III, II, IV. C) III, IV, I, II. D) III, IV, II. E) II, III, IV.arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forward
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