Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 18, Problem 14TYK
Summary Introduction
To review:
The evolution of heat stable DNA (deoxyribonucleic acid) polymerase enzyme to form stronger or weaker chemical attractions with each other.
Introduction:
DNA polymerase enzymes are used in polymerase chain reaction (PCR) for replication and elongation of DNA sequences. They bind to the single DNA strand and synthesize two identical strands. It is widely used in PCR analysis for the amplification of particular DNA fragments. They are heat-stable and are synthesized from thermophilic bacteria.
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Students have asked these similar questions
Which of the following experimental results was NOT evidence that DNA is the genetic molecule rather than proteins?
a.
When a virus was radioactively labelled, and the virus was allowed to infect a bacteria cell, radioactive virus DNA was found inside the bacteria cell while radioactive virus proteins were found outside the cell.
b.
Dead pathogenic bacteria cells combined with living nonpathogenic bacteria cells caused the creation of living pathogenic cells and thus the death of the host animal.
c.
Proteins are a class of macromolecules with the diversity and specificity needed for hereditary material.
d.
Nucleotide bases occur regularly, such that the number of adenines = number of thymines, and the number of guanines = number of cytosines.
A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe?
a. The reaction will work, but at a significantly slower rate.
b. The reaction will work, but the product will contain many undesired mutations.
c. The reaction will work, but amplify a region that was not his target.
d. The reaction will be completely unsuccessfu
If Avery, MacLeod, and McCarty had found that samples of heat-killed bacteria treated with RNase and DNase transformed bacteria, but that samples treated with protease did not, what conclusion would they have drawn?
a. Protease carries out transformation.
b. RNA and DNA are the genetic materials.
c. Protein is the genetic material.
d. RNase and DNAse are necessary for transformation
Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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- An explorer discovers a strange new species of plant and sends some of the plant tissue to a geneticist to study. The geneticist isolates chromatin from the plant and examines it with an electron microscope. She observes what appear to be beads on a string. She then adds a small amount of nuclease, which cleaves the string into individual beads that each contain 280 bp of DNA. After digestion with more nuclease, a 120bp fragment of DNA remains attached to a core of histone proteins. Analysis of the histone core reveals histones in the following proportions: H1 12.5% H2A 25% H2B 25% H3 0% H4 25% H7 (a new histone) 12.5% On the basis of these observations, what conclusions could the geneticist make about the probable structure of the nucleosome in the chromatin of this plant?arrow_forwardWhich of the following statements about nucleic acids is not correct? Select one: A. In DNA the distance between the strands of the double helix is relatively constant. B. The growing DNA polymer is synthesized in the 5' to 3' direction. C. The enzymes that catalyze DNA synthesis are known as DNA polymerases. D. RNA strands are synthesized in the 3' to 5' direction. E. Unlike DNA, RNA is easily cleaved because the 2'-OH group of the ribose is a nucleophile that catalyzes hydrolysis of the strand.arrow_forwardPlease answer the following question and explain Q1. If MacLeod and McCarty had accidentally contaminated their RNase enzyme with DNase, how would this have changed the conclusion that they made from their experiments? Pick the correct letter option A. They would have concluded that DNA is the only molecule required for genetic transformation. B. They would have concluded that DNA and protein are both required for genetic transformation. C. They would have concluded that RNA is the only molecule required for genetic transformation. D. They would have concluded that RNA and DNA are both required for genetic transformation. E. They would have concluded that proteins are the only molecules required for genetic transformationarrow_forward
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