Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 18, Problem 9TYK
Summary Introduction
Introduction:
Restriction fragment length polymorphism (RFLP) is a molecular technique which is used to identify the differences in DNA (deoxyribonucleic acid) sequences between individuals. The restriction endonuclease is used for cutting the nucleotides at different restriction sites on the DNA, which differentiate bands between two individuals after electrophoresis.
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Certain restriction endonucleases produce cohesive (sticky) ends. This means that they:
a.
stick tightly to the ends of the DNA they have cut.
b.
cut both DNA strands at the same base pair.
c.
make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding.
d.
cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content.
e.
cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.
The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA.
Select one:
True
False
Why?
Select one:
a. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are different.
b. It must use same restriction endonuclease so that the restriction sites are identical in both foreign and bacterial DNA.
c. It must use same restriction endonuclease so that the restriction sites are different in both foreign and bacterial DNA.
d. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are exactly the same.
RELPs:
A. are the same length for mutant and normal beta-globin alleles.
b. determine the sequence of bases in a DNA fragment.
c. have short, palindromic sequences of DNA in the middle.
D. are used as vectors.
e. are produced by reaction with restriction enzymes and are detected with PCR and gel electrophoresis.
Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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- What is the expected frequency of the restriction site 5′−GGCC−3′ in a genome? A. Once every 4 nucleotides. B. Once every 16 nucleotides. C. Once every 64 nucleotides. D. Once every 128 nucleotides. E. Once every 256 nucleotides.arrow_forwardWhat is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of human diseases? O a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one OC. The size of a band digested by specific restriction enzymes is different from the expected one O d. The DNA band detected by Southern blot is different from that by Northern blotarrow_forwardHindIII is a restriction endonuclease that recognizes a palindrome sequence. Predict which one is the HindIII recognition site: a. AAGGTT b. AAGCTT c. AGCCGA d. AGCGCA e. AGCAGCarrow_forward
- One would be correct in assuming that the restriction sites present in the multiple cloning site of the pUC18 plasmid will a. be the only instance of that restriction site in the plasmid b. appear at least once in another location in the vector. c. only include 4 bp restriction enzyme recognition sites. d. only be recognition sites that produce blunt ends when cleaved by the appropriate enzyme.arrow_forwardThe basis for DNase I footprinting is that the binding of a proteinto DNAa. prevents the DNA from being digested with a restrictionenzyme.b. enhances the ability of the DNA to be digested with a restrictionenzyme.c. prevents the DNA from being digested with DNase I.d. enhances the ability of the DNA to be digested by DNase I.arrow_forwardA more modern molecular technique to RFLP fingerprinting is called Amplified Fragment Length Polymorphisms (AFLPs). In AFLP analysis, restriction enzymes are again used to digest genomic DNA into multiple fragments. Next, adapters complementary to restriction site overhangs are ligated to the fragments using an enzyme called DNA ligase. These adapters are complementary to primers used to amplify the fragments using the polymerase chain reaction (PCR). Can you think of any potential benefits of AFLP analysis over RFLP? Explain your reasoning.arrow_forward
- Which of the following statements about restriction fragment length polymorphism is FALSE? Select one: O a. RFLPS have greatly increased the number of sites involved in mapping the human genome. b. RFLPS reflect the fact that molecular differences in alleles alter the site where restriction enzymes function. C. RFLPS can be used as a genetic fingerprint. d. RFLPS can be used to distinguish between identical twins. e. RFLPS are based on variations in alleles at the same locus.arrow_forwardIf you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know if the protein encoded by the cloned gene is expressed or not, which of the following methods is the right one to use? Select one: a. Northern blot b. Both Northern and Western blots c. Agarose gel with polyacrylamide d. Western blot e. Protein gel and northern blotarrow_forwardRestriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the following information can be gathered from the analysis of restriction maps? Select all that apply. a gene sequence b presence of mutations c nucleotide sequence d disease identificationarrow_forward
- A has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choicearrow_forwardOne would be correct in assuming that the restriction sites present in the multiplecloning site of the pUC18 plasmid willarrow_forwarda. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?arrow_forward
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