Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 18, Problem 4TYK

After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to:

a. amplify the DNA.

b. convert cDNA into genomic DNA.

c. convert cDNA into messenger RNA.

d. verify that the desired DNA sequence has been amplified.

e. synthesize primer DNA molecules.

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For each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.
The CRISPR method is used in  a.DNA sequencing. b.DNA fingerprinting. c.genome editing. d.the polymerase chain reaction.
Place the steps of sanger sequencing in order.A.  A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B.  ddNTPs bind and stop chain extension.C.  DNA fragments are separated by size through a capillary tube. D.  DNA polymerase copies the target region of template DNA.E.  The final nucleotide of each fragment is labeled with a fluorescent tag.
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