The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location and thus different cells end up with the DNA sequence in different positions.  A.    It has been shown that where the healthy gene integrates is heavily influenced by chromatin.  Why?

Q: Genes with highly similar sequence are often located adjacent one another in the genome. Gene…

A: Linked genes are genes that are tightly placed on the same chromosome and tend to inherit together.…

Q: What is a genomic library? O collection of sequences made of DNA fragments O collection of vectors…

A: The molecule that contains the genetic information necessary for an organism's growth and…

Q: Describe the difference between a transcriptional fusion and a translational reporter gene fusion.…

A: Transcription fusion means cloning the promoter of interest, upstream of any transcription unit,…

Q: linkage disequilibrium

A: a) Linkage:  Linkage is the phenomenon of certain genes staying together during inheritance…

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: The sequencing of entire genomes has made it possible to examine the level of gene expression in a…

A: The sequencing of the entire genome, from the small stretch of oligonucleotide probe, so that the…

Q: A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the…

A: if we cut the plasmid with XhoI and XbaI then we will get three fragments of following sizes  1000…

Q: You need to amplify a gene from a source DNA for cloning in any cloning vector, which enzymes will…

A: The method of DNA cloning is used to produce the multiple identical copies of the specific template…

Q: Which of these pairs of clinical applications of DNA repair mechanisms is not accurate? Group of…

A: To correct the DNA damage within cells, the cell uses various cellular repair mechanisms called DNA…

Q: When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on…

A: Polymerase chain reaction (PCR) is a frequently used approach for rapidly producing millions or even…

Q: . a. Mice engineered to block expression of the geneencoding the telomerase protein age at a…

A: Telomerase is an enzyme that is essential for the replication of chromosomal ends. It is a…

Q: a. What sequence information about a gene is lackingin a cDNA library?b. Can clones in a cDNA…

A: The cDNA or complementary DNA is derived from mRNA which is transcribed from genomic DNA (gDNA).  It…

Q: Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts…

A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: In a clinical context, doctors are evaluating a therapy for a new patient (say patient B) that they…

A: In the setting of assessing treatment for a modern patient (patient B) who may develop comparable…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: Microarrays can be used to determine relative levels of gene expression. In one type of microarray,…

A: Antibiotic resistance causes growth of various strains of bacteria even after the addition of…

Q: Fill in the Table  Broad Topic: Specific Topic: What is known: Experimental Question:…

A: This work explores single-cell level gene expression mistakes in Bacillus subtilis. The study looks…

Q: The MET oncogene is a growth factor receptor that is altered in many forms of cancer including lung…

A: Cancer is a fatal disease that has both physical and mental consequences for a person. Cancer can…

Q: magine you are working in a lab that is developing a novelty rose that smells like a stinkhorn…

A: Introduction Recombinant DNA technology is advanced technology to produce hybrids plants and animals…

Q: You are interested in finding out in which organ GTF2H5 is highly expressed experimentally. Describe…

A: Recombinant DNA also written as rDNA is formed by joining or combining the DNA from different…

Q: A. Which genes could be used to monitor the process of the disease? B.  If you worked for a drug…

A: By collecting mRNA samples from patients over the course of four phases, we may analyze the…

Q: Describe the mechanisms in which DNA is used to generate protein.  Reflect on the key points in the…

A: Deoxyribonucleic acid is a molecule composed of two polynucleotide chains that coil around each…

Q: To identify the following types of genetic occurrences, would acomputer program use sequence…

A: Introduction  With the advancements of Bioinformatics tools and sequencing techniques we can easily…

Q: Should the exposome field focus exclusively on measuring chemicals derived from exogebeous sources…

A: The term "exposome" describes all of an individual's lifetime environmental exposures, including…

Q: Itransferases and analyze the cells after they at do you expect to find in the promoter of gene X?…

A: Hematopoietic stem cells (HSCs) resides within the bone marrow, which serves as niche for the…

Q: Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind…

A: It is difficult for one to to find out gene of interest out of a complete genome. However, a given…

Q: a. Replicate this sense strand to create a double-stranded DNA helix. Write your answers in CAPS…

A: DNA replication is a phenomenon in which DNA make itself using Enzyme DNA Polymerase . It occurs in…

Q: The restriction digests of the cloned Drosophila gene can provide direct visible evidence of a…

A: We can use the technique of Southern blotting to detect and isolate the DNA fragment with the Gene…

Q: 6. Suppose a particular gene is required for early development and also later for development of a…

A: ANSWER;- The Gal4 (galactose) gene articulation is directed by Gal80. The Gal4 gene protein…

Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

A: Answer. Blotting describes the immobilization of sample nucleic acids/proteins onto a solid support.…

Q: RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with…

A: Biotechnology is the part of applied science that utilizations living organic entities and their…

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: The expression of a transgene depends on its site of integration, state of differentiation of target…

Q: STR markers: are point mutations detectable by DNA sequencing are variations in the number of…

A: STR markers, also known as short tandem repeat markers, are regions of DNA that contain a pattern of…

Q: On the image, you can see the results of a DNA microarray used for cancer prognosis, where tissue…

A: DNA microarray is the molecular biology technology that is used to detect the expression of multiple…

Q: a. Describe two ways you could potentially make atransgene that would inhibit the function of a…

A: A transgene is a gene, which is transferred from one organism to another organism naturally or…

Q: The abundance of three mRNA transcripts, transcripts A, B, and C, was measured by real-time PCR. The…

A: In molecular biology, understanding the abundance of specific mRNA transcripts and their…

Q: A patient is diagnosed with lung cancer using imaging. To establish the best treatment option,…

A: Since you've asked multiple questions, we're only answering the first three for you. If you want any…

Q: Several DNA coding for different proteins, CRISPR, or siRNAs against different genes were expressed…

A: The given experimental setup involves the expression of various proteins and treatments using siRNAs…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.