The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location and thus different cells end up with the DNA sequence in different positions.  A.    It has been shown that where the healthy gene integrates is heavily influenced by chromatin.  Why?  B.     The same gene integrated into different locations in the genome will be expressed differently.  Why?  C.     Over time the expression of an integrated gene will change, usually decreasing.  Describe how you think the cell downregulates expression of an integrated gene.

Q: Describe the difference between a transcriptional fusion and a translational reporter gene fusion.…

A: Transcription fusion means cloning the promoter of interest, upstream of any transcription unit,…

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: Mutant C has had the first 5 base pairs deleted (position 1-5). Does this mutation change the…

A: Translation is the process which is responsible for synthesis of protein from the mRNA.

Q: The sequencing of entire genomes has made it possible to examine the level of gene expression in a…

A: The sequencing of the entire genome, from the small stretch of oligonucleotide probe, so that the…

Q: Occurred in her cells? (Unfortunately her parents have passed away.) I think these genes are…

A: CRISPR stands for Clustered regularly interspaced short palindromic repeats. It is a system of…

Q: Which of these pairs of clinical applications of DNA repair mechanisms is not accurate? Group of…

A: To correct the DNA damage within cells, the cell uses various cellular repair mechanisms called DNA…

Q: The figure below summarizes the results of an experiment evaluating the role of Ku80 gene DNA…

A: The gene knockout is performed for the detection of the role of a specific gene. The elimination of…

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: Regulatory regions for genes can also be cloned using molecular-genetic techniques, allowing…

A: Yes, Regulatory regions of genes can be cloned using molecular genetic techniques for studying gene…

Q: The figure below shows differential methylation patterns for various genes in samples of 2 embryonic…

A: The process by which methyl groups are added to the DNA sequences is called DNA methylation.…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: SCENARIO: You are studying a disease caused when the gene called BMP5 is not transcribed in…

A: First of all, we put the reporter gene (GFP) in the diseased cell in which BMP5 is not transcribed.…

Q: Microarrays can be used to determine relative levels of gene expression. In one type of microarray,…

A: Antibiotic resistance causes growth of various strains of bacteria even after the addition of…

Q: Researchers create a recombinant DNA molecule in which the coding sequence for GFP is inserted…

A: Recombinant DNA is the DNA that is formed by insertion of gene of interest into the original DNA.…

Q: Concentration (%) A researcher is studying environmental conditions that cause the expression of…

A: We analyze the correlation between pH values and active repressor concentration to identify the…

Q: Suppose that an investigative team conducted an RNA-Seq experiment on mouse liver cells. They found…

A: DNA sequence segments that straddle the start and stop codons are known as open reading frames…

Q: Illustrate and label the 12 cranial nerves.

A: Cranial nerves are pairs of nerves (set of 12 paired) that arises from the brain and connect…

Q: Would the viral insertion of a proto-oncogene into a region of genomic DNA with non-acetylated…

A: Acetylation reduces the affinity between histones and DNA by removing positive charges. Thus,…

Q: You are using Drosophila as a model system to investigate eye development. You do positional cloning…

A: In order to isolate a mutant gene in any organism, one would need to first identify the desired…

Q: a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter…

A: Transcription - The process by which the information on one strand of DNA is copied to mRNA is…

Q: You are interested in finding out in which organ GTF2H5 is highly expressed experimentally. Describe…

A: Recombinant DNA also written as rDNA is formed by joining or combining the DNA from different…

Q: Describe error prone polymerases and the process of translesion synthesis (TLS). In regards to tumor…

A: Translesion synthesis (TLS) is a method of overcoming stopped replication in which particular…

Q: Describe the mechanisms in which DNA is used to generate protein.  Reflect on the key points in the…

A: Deoxyribonucleic acid is a molecule composed of two polynucleotide chains that coil around each…

Q: The reason LacZ is frequently used as a reporter gene is: a. The lacZ gene product binds to the…

A: LacZ is a gene of lac operon which codes for β-galactosidase. The reporter genes cause a visible…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: RNAi is a method to assess........ gene expression    gene sequence  gene function  Chromosomal…

A: A gene is a functioning heredity unit made up of DNA, which provides instructions for the creation…

Q: You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane…

A: Reverse transcription enzyme chain reaction (RT-PCR) may be a variation of normal PCR that involves…

Q: From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is…

A: Transgenic approach has been proved to be very useful in studying physiological function of a…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.