In a microarray experiment, if you label cDNA from Tissue #1 with a red color and cDNA from tissue #2 with a green color and perform a microarray, predict the following gene expression patterns? : A dot that is only red? A dot that is only green? A dot that is only yellow? A dot that is black?

Q: How do you clone a 4 kb promoter from a genomic cell preparation?

A: Genome is the complete set of genetic material present in the nucleus of a cell. It houses all the…

Q: How do we use genomic or cDNA libraries to find our gene of interest in a forward genetic screen?

A: Reverse genetics:It is an approach in molecular genetics. It is used to help in understanding the…

Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?

A: A knockout mouse, or knock-out mouse, is a genetically modified mouse in which researchers have…

Q: You complete a comparative transcriptomics experiment, where RNA from a plant sample grown in sandy…

A: The researchers compared the gene expression differences between plants cultivated in silty soil and…

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: DMC1 SPS1 DIT1 SPS100 fold repressed >20 1:1 fold induced x10x >20

A: The given question states three genes as named X, Y, Z and their transcriptional responses over time…

Q: How the amplification of other genomic methods such as optical mapping helps to overcome the…

A: The amplification of other genomic methods like optical mapping  has definitely improved the…

Q: ain A: wild type........ .CT value of 15 ain B: has mutation X in the promoter region........CT…

A: Quantitative reverse transcription polymerase chain reaction, also called RT-qPCR, is a molecular…

Q: What is the difference of intragenic and intergenic regions? Please explain with drawing.

A: In the intricate tapestry of the genome, the terms "intragenic" and "intergenic" encapsulate…

Q: What are ‘Selectable marker’? What is their use in genetic engineering?

A: Genetic engineering is a technique to manipulate the genetic content of an organism resulting in…

Q: Besides gene expression, what else can be assayedusing gene chips?

A: DNA microassays or DNA chips contain thousands of DNA sequences of individuals arrayed at a great…

Q: In yeast, you have sequenced a piece of wild-type DNA and it clearly contains a gene, but you do not…

A: To find the mutated genes one must code all the amino acid of the genetic modified yeast and normal…

Q: What is knockdown gene analysis? How is this different from knockout analysis?

A: Gene knockdown is a method in which temporarily stop or decrease the expression of one or more…

Q: out transformation requires from 105 to 124 genes, collectively termed the com regulon. The com…

A: Transformation is a process in which the DNA is transformed or changed by taking up the DNA that is…

Q: You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker…

A: First of all, Western Blot is a technique which is used to detect the expression of protein from…

Q: What is the difference between a genetic map and a physical map? Which generally has higher…

A: Genes are the basic structural and functional unit of heredity. They carry coded genetic information…

Q: What are potential uses of CRISPR-Cas9 Technologies? Silence genes to study their function Identify…

A: CRISPR-Cas9 is a gene editing tool that allows scientists to precisely target and cut specific DNA…

Q: Discuss the major challenges when using microarray techniques to study differential gene expression.…

A: Microarray is a powerful laboratory technique used in genomics and molecular biology to…

Q: RNAi is a method to assess........ gene expression    gene sequence  gene function  Chromosomal…

A: A gene is a functioning heredity unit made up of DNA, which provides instructions for the creation…

Q: How will this modified cro protein interact with the three OR sites, and how would cro expression be…

A: Bacteriophages are viruses that infect bacteria. Like other viruses bacteriophages are also made up…

Q: How would you use a ChIP (chromatin immunoprecipitation) to show that when RNA pol II is bound at…

A: Chromatin immunoprecipitation is a technique used for the investigation of DNA (deoxyribonucleic…

Q: When considering the cause of the disease in a patient who is the child of an inbred couple, it was…

A: 1. The given situation describes a child, born to an inbred couple, who is suffering from a…

Q: Explain the experimental advantage of genetic mapping?

A: Gene mapping was discovered by Alfred H. Sturtevant. He discovered this in order to identify the…

Q: By whole-exome sequencing, you have identified an early termination mutation in KLHL4 in a human…

A: "Hi, since you have posted more than one question, we will be answering the first two for you.…

Q: 0What typé of nucleic acid and from what species would the scientist use to begin construction of…

A: Drosophila Cryptochrome -- Drosophila Cryptochrome is a blue - light sensitive protein which…

Q: How can deletion experiments within gene sequences help identify important DNA sequences for the…

A: DNA:  DNA (Deoxyribonucleic Acid) is a molecule that carries genetic information in most living…

Q: How have GWAS's changed the field of genomics and what do they allow that other methods do not? What…

A: Introduction :- Genomics is a field of biology that focuses on the study of the structure, function,…

Q: You are studying celiacs disease and have identified a potential gene for the disease using linkage…

A: Genetics is the study of the heredity, expression of genes and control of phenotypes. There are…

Q: Several DNA coding for different proteins, CRISPR, or siRNAs against different genes were expressed…

A: The given experimental setup involves the expression of various proteins and treatments using siRNAs…

Q: When performing QPCR to study differential gene expression, what is the role of SYBR green? What is…

A: Introduction :- SYBR Green is a cyanine dye used in molecular biology studies as a nucleic acid…

Q: When doing the virtual DNA microarray analysis, which color (red, green or yellow) would the spot be…

A: DNA microarray analysis is a laboratory tool used to detect the expression levels of several genes…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.