0What typé of nucleic acid and from what species would the scientist use to begin construction of her genomic DNA library? b. From what tissue would she isolate this nucleic acid? =. What type of reagent would the scientist use to cut the genome into appropriately sized fragments?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Assume a scientist is living back in the dark ages of the 1970s - before
any human genomes had been sequenced - even before PR methodology had been
established - and decides she wants to isolate a genomic DNA clone from a mosquito that
codes for a cryptochrome, a blue light photoreceptor involved in setting the circadian clock in
insects. Her friend in the next lab has actually isolated the cDNA clone coding for cryptochrome
from the fruitfly Drosophila melanogaster. The scientist decides she will begin her experiment by
making a mosquito genomic library. Given this information, answer the following questions:
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