You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located? Group of answer choicesAt the 5’ end of the gene and the C-term of your protein of interestAt the 5’ end of the gene and the N-term of your protein of interestAt the 3’ end of the gene and the C-term of your protein of interestAt the 3’ end of the gene and the N-term of your protein of interest **Question 4** **2 pts**You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located?- ○ At the 5' end of the gene and the C-term of your protein of interest- ○ At the 5' end of the gene and the N-term of your protein of interest- ● At the 3' end of the gene and the C-term of your protein of interest- ○ At the 3' end of the gene and the N-term of your protein of interestThe correct answer is selected with a blue dot.

When analyzing cells expressing a fusion gene encoding a FLAG-tagged protein of interest, it's…

Answered: You are analyzing cells that express a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located?

Group of answer choices

At the 5’ end of the gene and the C-term of your protein of interest

At the 5’ end of the gene and the N-term of your protein of interest

At the 3’ end of the gene and the C-term of your protein of interest

At the 3’ end of the gene and the N-term of your protein of interest

**Question 4**
**2 pts**

You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located?

- ○ At the 5' end of the gene and the C-term of your protein of interest
- ○ At the 5' end of the gene and the N-term of your protein of interest
- ● At the 3' end of the gene and the C-term of your protein of interest
- ○ At the 3' end of the gene and the N-term of your protein of interest

The correct answer is selected with a blue dot.

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