The practical limit for the number of different RNA sequences screened in a SELEX experiment is 10¹5. Suppose you are working with oligonucleotides 36 nucleotides long. How many sequences exist in a randomized pool containing every sequence possible? total number of sequences: What percentage of these can a SELEX experiment screen? percentage of RNA molecules screened in SELEX experiment: Suppose you wish to select an RNA molecule that catalyzes the hydrolysis of a particular ester. From what you know about catalysis, which SELEX strategy might allow you to select the appropriate catalyst?

Q: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…

A: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI: 11…

Q: Using the nucleotide sequence of an accession number (NM_019121) what is the translated amino acid…

A: Proteins are made up of amino acids. DNA is the genetic material that codes for the amino acids that…

Q: Biochemistry: Site-directed mutagenesis, in which individual amino acid residues are replaced with…

A: Enzyme activity is used to assess the catalytic activity of an enzyme. One enzyme unit (U) is the…

Q: Consider the proteins in Figure 1.  Assume they are treated with chymotrypsin to cut them into…

A: Chymotrypsin:  It is a proteolytic enzyme that cuts the protein at a specific point. Proteolytic…

Q: In describing the triplet binding assay, an undergraduate with limited knowledge of translation…

A: Every three nucleotides in a row count as a triplet in the genetic code and codes for a single amino…

Q: Christian Anfinsen showed that the enzyme Ribonuclease (RNase) is completely inactivated at high…

A: In the 1950s, Christian Anfinsen performed a series of studies. He discovered that all the…

Q: The Shine-Dalgarno Sequence is used in bacteria eukaryotes both

A: The nucleotide segment at the 3' terminal of 16S ribosomal RNA (rRNA) has several pyrimidines with a…

Q: The following RNA sequence represents a small messenger which can be translated in a prokaryotic…

A: The first step in translating an RNA sequence into a protein is to divide the sequence into codons,…

Q: Consider the following coding 71 nucleotide DNA template sequence (It does not contain a…

A: In cell protein formed according to the sequence on mRNA. mRNA is formed from DNA sequence by the…

Q: The disease cystic fibrosis is the result of a mutation in the gene that encodes the cystic fibrosis…

A: A codon is a DNA or RNA trinucleotide sequence encoding a particular amino acid or signaling the…

Q: Select one: O Met-Cys-Ala-Thr-Tyr---- O Met-Cys-Cys-Thr-Tyr--- O Met-Ala-Ala-Thr-Tyr---- O…

A: Aminoacyl tRNA synthetase are enzymes of extreme importance during the process of protein synthesis…

Q: Translation work is an essential step for protein synthesis. In order for the protein to be…

A: The expression of genes includes two major process: transcription and translation. In transcription,…

Q: SDS-PAGE and Agarose gel electrophoresis can both be used to separate proteins or protein- complexes…

A: Given Values: SDS-PAGE bands = 86 kD and 136 kD Agarose gel band = 222 kD and 444 kD

Q: Need help:, The rRNAs are isolated from the large subunit of a bacterial ribosome and separated by…

A: Ribosomal RNA (rRNA) is a fundamental molecule found within cells, specifically in cellular…

Q: Imagine that you repeat the tRNA Selection experiment with modifications as follows: 1. Synthesize…

A: We know that the amino acid sequence of a polypeptide synthesized with respect to an mRNA is based…

Q: . The following data represent the base compositions of double-stranded DNA from two different…

A: A gene is the essential physical and functional unit of heredity. They are comprised of DNA…

Q: The ribonucleotides C and G we're mixed together with C two times more abundant than G. The…

A: Introduction: The synthesis of a protein or a polypeptide chain in a living cell is known as…

Q: You gathered your data from Lab 4, and found the following values: Total Activity of lysozyme in…

A: Total Activity of lysozyme in HEW: 14 units  Total amount of protein in HEW: 70 mg  Total Activity…

Q: electrostatic dispersion negative C-terminus positive positively negatively N-terminus is…

A: Proteins are biomolecules made up of repeating monomeric units known as "amino acids", the sequence…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.