In a genomic analysis looking for a specific disease gene,one candidate gene was found to have a single-base-pairsubstitution resulting in a nonsynonymous amino acidchange. What would you have to check before concluding that you had identified the disease-causing gene?

Q: Recombination signal sequences are conserved heptamer and nonamer sequences that flank the V, J, and…

A: Introduction: RAG 1 & 2 are the recombination activating genes that encode a portion of a…

Q: Although DNA transposons are abundant in the genomes of multicellular eukaryotes, class 1 elements…

A: Transposons are called jumping genes they change their position within a Genome.

Q: Price et al. (1999. J. Bacteriol. 181: 2358–2362) conducted a genetic study of the toxin transport…

A: Missense mutation- When one or more amino acids are replaced by the other amino acid.

Q: In the Avery, McCarty and McCleod experiment, DNA was identified as the heritable material because,…

A: A long time back it was unknown which the genetic material among protein, RNA, and DNA. It was later…

Q: Following a dye terminator DNA sequencing reaction using 2',3'-dideoxynucleotide triphosphates…

A: Dye terminator DNA sequencing is the modern version of Sanger sequencing. It uses…

Q: A very common molecular biology research method is to analyze cell or tissue homogenates by…

A: In molecular biology research, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting,…

Q: Why did we obtain the sequence from the forward primers separate from the reverse primer?

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that passes genetic information from one…

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: DNA from 100 unrelated individuals from one population of Chinook salmon were amplified at a single…

A: A population is said to be in Hardy Weinberg equilibrium if it satisfies 2 equations; p+q+r=1 and…

Q: You obtain the DNA sequence of a mutant of a 2-kb gene in which you are interested and it shows base…

A: the mutation is the change or error that is caused in the chromosome it is of a different type 1.…

Q: You are studying the tryptophan synthetase gene that Yanofsky also examined to determine the…

A: Charles Yanofsky and colleagues in 1964 discovered that nucleic acid sequences and the translated…

Q: Transposon mutagenesis

A: Transposons: These are also known as jumping genes. These are the segments of DNA which can move or…

Q: For the following sequence please design an 18 base pair forward primer.  ATG TCA AAA GCT GTC GGT…

A: Introduction :  Primers are short oligonucleotides that are chemically synthesised and complementary…

Q: If I'm understanding your explanation correctly, do you mean that option D "At the 3’ end of the…

A:

Q: you wanted to design two primers to PCR amplify a gene from an organism that has 4 chromosomes, each…

A: In PCR that is, polymerase chain reaction two primers are needed  to hybridize with the sample DNA…

Q: The xyz gene product, encoded by a new viral strain, has coresponding human homologs. Blast analyses…

A: Scoring matrix  Scoring matrix measures the similarity between two characters in sequence alignment.…

Q: Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map…

A: Horizontal gene transfer (HGT) is the exchange of genetic information across organisms, which…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.