Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant Brassica. Wildtype DNA 3’ CTT AAT GCT CCG AAT CCA 5’ template strand 5’ GAA TTA CGA GGC TTA GGT 3’ non-template strand A new strain (Strain X) of Brassica is identified with the same region of the gene coding for protein Y: 3’ CTT AAT GCT GCG AAT CCA 5’ template strand 5’ GAA TTA CGA CGC TTA GGT 3’ non-template strand Compare the sequence of Wildtype with Strain X DNA, and note the following: Whether there is a mutation. If there is a mutation, what is the type of mutation (be as specific as possible) and explain the rationale for your decision. Assuming this is the only difference between the Wildtype and Strain X, describe the potential impact of the mutation on the structure and function of the protein.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant Brassica.
Wildtype DNA
3’ CTT AAT GCT CCG AAT CCA 5’ template strand
5’ GAA TTA CGA GGC TTA GGT 3’ non-template strand
A new strain (Strain X) of Brassica is identified with the same region of the gene coding for protein Y:
3’ CTT AAT GCT GCG AAT CCA 5’ template strand
5’ GAA TTA CGA CGC TTA GGT 3’ non-template strand
Compare the sequence of Wildtype with Strain X DNA, and note the following:
- Whether there is a mutation.
- If there is a mutation, what is the type of mutation (be as specific as possible) and explain the rationale for your decision.
- Assuming this is the only difference between the Wildtype and Strain X, describe the potential impact of the mutation on the structure and function of the protein.
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