In a transformation experiment involving a wild type bacterial strain with a recipient strain with mutations in genes f, g, h and i pairs of genes were analyzed for co-transformation with the following results: Gene Pair Co-transformation g+ i+ ft i+ yes no f+ h+ yes ft g+ no g+ h+ h+ i+ no yes What is the linear order of these genes relative to each other?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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g, h and i pairs of genes were analyzed for co-transformation with the following results:
Gene Pair
Co-transformation
g+ i+
f+ i+
yes
no
f+ h+
Уes
f+ g+
no
g+ h+
h+ i+
no
уes
What is the linear order of these genes relative to cach other?"
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