The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near the centromereof the X chromosome
The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.
For example, let’s consider the β-globin gene, which is
found on human chromosome 11. A probe complementary to the
β-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detect
where the β-globin gene is located within a set of chromosomes. Because
the β-globin gene is unique and because human cells are diploid
(i.e., have two copies of each chromosome), a FISH experiment
shows two bright spots per cell; the probe binds to each copy of
chromosome 11. What would you expect to see if you used the
following types of probes?
A. A probe complementary to the Alu sequence
B. A probe complementary to a tandem array near the centromere
of the X chromosome
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