Homework Help is Here – Start Your Trial Now!
Science
Biology
You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa 40 kDa 40 kDa - a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?

You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa 40 kDa 40 kDa - a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa -40 kDa 40 kDa a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? (S c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?
Transcribed Image Text:You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa -40 kDa 40 kDa a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? (S c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genomics
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS