You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa 40 kDa 40 kDa - a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?
You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa 40 kDa 40 kDa - a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:You observe the following:
Your
Your
friend's
Gel
Gel
120 kDa
100 kDa
120 kDa
100 kDa
-80 kDa
80 kDa
-40 kDa
40 kDa
a. You and your friend analyzed the same protein sample in your gels. Why are
your results different? Explain briefly which gel is the correct representation of
the SDS-PAGE & Western blot, and why?
b. Explain briefly the size of protein observed in the chromatography column and
the Western blot. Are they different or the same, why? (S
c. If the primary antibody to Protein A was made in a mouse, describe the details
of the secondary antibody used in the Western blot analysis?
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