When performing QPCR to study differential gene expression, what is the role of SYBR green? What is the most correct answer? SYBRgreen intercalates proportionally and efficiently with double stranded DNA and can therefore be used to quantify it SYBRgreen intercalates proportionally and efficiently with nucleic acids and can therefore be used to quantify them. SYBRgreen intercalates more efficiently and proportionately with single stranded DNA and can therefore be used to quantify cDNA (a direct product of mRNA). SYBRgreen intercalates proportionally efficiently with messenger RNA and can therefore be used to quantify it.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
When performing QPCR to study differential gene expression, what is the role of SYBR green?
What is the most correct answer?
-
SYBRgreen intercalates proportionally and efficiently with double stranded DNA and can therefore be used to quantify it
-
SYBRgreen intercalates proportionally and efficiently with
nucleic acids and can therefore be used to quantify them. -
SYBRgreen intercalates more efficiently and proportionately with single stranded DNA and can therefore be used to quantify cDNA (a direct product of mRNA).
-
SYBRgreen intercalates proportionally efficiently with messenger RNA and can therefore be used to quantify it.
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