the experiment was repeated many times to improve the precision of the measurements O global analysis with wild type DNA and a fragment bearing a 5 base-pair insertion between the sites the intrinsic energy of binding for sites A and sites B were first extracted for a DNA fragment with a 5 bp insertion between the two sites, and then those parameter values were used as known quantities in analysis of the data for wild type DNA template

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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In the example two site system for DctD₂ binding to the dctA enhancer element, what was done to
sharpen the error surface for the wild type DNA fragment (plotted on the left), giving the much
more informative plot on the right?
UPOWRA
Variance of Fit vs Free Energy for Parameters
Gee's Free Energy
PERSOALAN
BOXE
4.00
2005
0:02
2015
6:01
1.005
44
20
Verlance of Fit us Free Energy Paramete
-16
G's free g
O the experiment was repeated many times to improve the precision of the measurements
O global analysis with wild type DNA and a fragment bearing a 5 base-pair insertion between the sites
the intrinsic energy of binding for sites A and sites B were first extracted for a DNA fragment with a 5 bp
insertion between the two sites, and then those parameter values were used as known quantities in analysis
of the data for wild type DNA template
Transcribed Image Text:In the example two site system for DctD₂ binding to the dctA enhancer element, what was done to sharpen the error surface for the wild type DNA fragment (plotted on the left), giving the much more informative plot on the right? UPOWRA Variance of Fit vs Free Energy for Parameters Gee's Free Energy PERSOALAN BOXE 4.00 2005 0:02 2015 6:01 1.005 44 20 Verlance of Fit us Free Energy Paramete -16 G's free g O the experiment was repeated many times to improve the precision of the measurements O global analysis with wild type DNA and a fragment bearing a 5 base-pair insertion between the sites the intrinsic energy of binding for sites A and sites B were first extracted for a DNA fragment with a 5 bp insertion between the two sites, and then those parameter values were used as known quantities in analysis of the data for wild type DNA template
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