For your experiment, you want to use a plasmid which is 8 kb long (see below). To digest the plasmid, you will only use the enzyme called EcoRI. After the digestion, you run the DNA on a gel which separates the DNA by size. What fragments do you expect after the digestion (assume every plasmid was cut)? O 8 kb O 1kb, 2kb and 5kb O 3kb and 5kb O2 kb and 6 kb HindIll, EcoRI 0 kb (8 kb) Plasmid X (8 kb) HindIll 2 kb EcoRI 3 kb
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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