From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is overexpressed in human cancer cells. You want to know if this gene is required for cancer cells to survive, but from your efforts at making a traditional gene knock out, you have found that it is required for embryonic development and can't get to the point of doing a cancer experiment. a. How can you redesign the knockout to get past embryonic lethality? b. How would you then use this mouse line to test if ABCG2 is needed for cancer survival?

From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is overexpressed in human cancer cells. You want to know if this gene is required for cancer cells to survive, but from your efforts at making a traditional gene knock out, you have found that it is required for embryonic development and can't get to the point of doing a cancer experiment. a. How can you redesign the knockout to get past embryonic lethality? b. How would you then use this mouse line to test if ABCG2 is needed for cancer survival?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy

Section: Chapter Questions

Problem 12QP: In selecting target cells to receive a transferred gene in gene therapy, what factors do you think...

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Retinoblastoma is an extremely rare cancer of the retina in the eye. The disease mainly affects children up to the age of 5 years because it can only occur while the nerve precursor cells are still dividing. In its nonhereditary form, a tumor usually occurs in only one eye; in its hereditary form, multiple tumors develop in both eyes.To explore the basis for these differences, a cDNA clone of the Rb gene was used to probe the structure of the gene in cells from normal individuals and from individuals with nonhereditary or hereditary retinoblastoma. As shown in the figure Part A, normal individuals have four restriction fragments (A, B, C, and D) that hybridize to the Rb cDNA probe, indicating that each restriction fragment encodes at least one Rb exon. Samples from fibroblasts and tumor cells of affected persons show some differences in the patterns of hybridization, with some bands missing entirely and some bands present at half intensity. The order of the restriction fragments in the…
In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?
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