What are site-recombinases? Describe in detail how cre-recombinase can be used to decipher the roles of specificgenes and proteins in complex multicellular organisms?Explain how the cre-lox system can be used to examinethe role of a particular gene in a specific type of tissue?

Genetic recombination is the transfer of genetic material between organisms, resulting in offspring…

Answered: What are site-recombinases? Describe in…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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When performing QPCR to study differential gene expression, what is the role of SYBR green? What is the most correct answer? SYBRgreen intercalates proportionally and efficiently with double stranded DNA and can therefore be used to quantify it SYBRgreen intercalates proportionally and efficiently with nucleic acids and can therefore be used to quantify them. SYBRgreen intercalates more efficiently and proportionately with single stranded DNA and can therefore be used to quantify cDNA (a direct product of mRNA). SYBRgreen intercalates proportionally efficiently with messenger RNA and can therefore be used to quantify it.
How can these designed nucleases modify the sequence of genomic DNA?
Explain how site-directed mutagenesis can be used to produce an altered protein in bacterial cells.
https://www.khanacademy.org/science/biology/biotech-dna-technology/dna-cloning-tutorial/a/overview-dna-cloning That is the link for the example ^
How do you design a reporter assay for Serpina1?
You want to clone a eukaryotic gene and express the corresponding protein in yeast. However, the protein typically localizes within mitochondria. How will you perform your gene cloning so that the protein is secreted from the cell, rather than localized within yeast mitochondria?
In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true: I. Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype II. Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type) III. Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype IV. Injection of the white experimental transgene in the rosy mutant…
Gene expression can be disrupted by techniques such as homologous recombination and RNA interference. What is the functional difference between these two methods in terms of the ultimate effect on gene expression? Why might homologous recombination or an alternative genome-editing method (e.g., CRISPR/Cas9) be preferred over RNA interference?
If you wanted to analyze the size and abundance of the HOAP protein in an extract from a Drosophila animal that you think may be mutant for the HOAP gene, what method could you use to target your analysis specifically to the HOAP protein in that extract?
You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located? Group of answer choices At the 5’ end of the gene and the C-term of your protein of interest At the 5’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the C-term of your protein of interest At the 3’ end of the gene and the N-term of your protein of interest
The occurrence of multiple transposons within the genome oforganisms has been suggested as a possible cause of chromosomalrearrangements such as deletions, translocations, and inversions.How could the occurrence of transposons promote these types ofstructural rearrangements?
Explain Constructing transgenic Drosophila by P element transformation?

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What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?