You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located? At the 5’ end of the gene and the C-term of your protein of interest At the 5’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the C-term of your protein of interest

Q: As part of a project investigating potential new drug targets in the fight against malaria, you are…

A: Introduction:-  Truncation of protein:- is meant by the proteolysis or structural gene manipulation…

Q: If a gene contains 2 introns and 3 exons, explain and draw what it would look like in an R loop…

A: R-loop is a structure containing 3 strands of nucleic acid and is all a hybrid of DNA: RNA with…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: A mutant is found that has the following protein sequence in that part of the protein: Tyr Lys Gly…

A: Amino acids are represented by codon. Each codon is made up of three nucleotide bases. A codon can…

Q: For a coding gene, purifying (negative) selection will result in more synonymous (silent) site…

A: Purifying selection is the most predominant form of natural selection or negative selection. As it…

Q: Suppose that the diagram below represents the genomic organization of an enzyme involved in eye…

A: Transcription is the process by which  RNA transcript is formed from DNA. Various type of RNA are…

Q: When comparing evolutionary similarities between different genes within a gene family, it is usually…

A: Genes are the basic units of inheritance in living organisms. They are passed down from parents to…

Q: You are interested in studying a unique protein found in a rare wildflower found in the desert…

A: The objective of the question is to understand the changes brought about by a mutation in a protein…

Q: Part A The bacterial gene little protein (lilP) makes a small protein of 11 animo acids (AA) in…

A: Introduction :  The translated form of the DNA sequence is the amino acid sequence. The DNA sequence…

Q: You construct a translational reporter gene fusion of your favourite gene. How can you assess…

A: Translational Gene Fusion is employed to live protein localization by quantifying promoter activity.…

Q: Consider a genome whose length is 1000 bp. "Shotgun" sequencing techniques are applied to the…

A: Shotgun sequencing is an important tool in modern genomics that allows researchers to interpret the…

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: You obtained the sequence of the frog gene X you amplified in Question #16 through a process called…

A: A linear polymer of nucleotides is called as nucleic acid. DNA (Deoxyribonucleic acid) and RNA are…

Q: You isolate a nuf gene mutant and determine the base sequence of the mutant mRNA:  What is the…

A: The mRNA (messenger ribonucleic acid) is the transcript molecule synthesized from a gene by the…

Q: You obtain the DNA sequence of a mutant of a 2-kb gene in which you are interested and it shows base…

A: the mutation is the change or error that is caused in the chromosome it is of a different type 1.…

Q: Shown below is an R loop prepared for electron microscopy by annealing a purified eukaryotic…

A: An exon is any part of a gene that will encode a part of the final mature RNA produced by that gene…

Q: A plant geneticist was examining the inversion mutation XI 501 in barley where a segment of DNA is…

A: Any alteration occurring in the DNA sequence of an organism is known as mutation.

Q: A scientist used a transgenic strain of C. elegans into which a gene (gfp) for a green fluorescent…

A: Introduction :- Transgenic organism's are those in which a foreign gene is introduced and this…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: The E. coli genome contains approximately 4639 kb. (a) How many copies of the 6-bp recognition…

A: E.coli is a type of bacteria that normally lives in the intestine. It is also found in the gut of…

Q: When an EcoR1 fragment, which represents the coding region of a human gene X, is cloned into the…

A: Fusion proteins are created by combining two or more genes into a single gene. GST is a common…

Q: that the

A: Polypeptides, or proteins, are chemical compounds composed up of amino acids. They're big,…

Q: The consensus sequence of the protospacer adjacent motif (PAM) sequence in bacterial strain A is…

A: For Cas9 to work, it additionally requires a particular protospacer adjacent motif (PAM) that…

Q: The genetic alteration responsible for sickle-cell anemia in humans involves: a transition mutation…

A: sickle cell anaemia is caused by inherited abnormal hemoglobin that causes the sickling of red blood…

Q: DNA sequencing can help us to identify mutations withingenes. The following data are derived from an…

A: Any change in the sequence of DNA is termed as mutation, it can be due to physical or chemical…

Q: DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain…

A: Co transformation means that the two genes are transformed simultaneously. Is two genes are co…

Q: Your colleague wants to align the complete sequence of the collagen gene from humans to the…

A: Homologous genes are genes that share a common ancestry. When two species share a common ancestor,…

Q: RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with…

A: Biotechnology is the part of applied science that utilizations living organic entities and their…

Q: Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up…

A: Answer. The majority of eukaryotic genes contain non-coding DNA. The genes tend to have a split…

Q: The coding sequence of genes that specify proteins with the same function in related species…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Duchenne muscular dystrophy is caused by a mutation in a gene that comprises 2.5 million base pairs…

A: Dystrophin gene the largest known human gene provides instructions for making a protein called…

Q: this is what i have said about this image so far, what else can be said aswell including the raw…

A: The raw count data presented in this comprehensive gene expression analysis offers a quantitative…

Q: Select all that apply: A SNP (single nucleotide polymorphism) is similar to an STR (short tandem…

A: SNP and STR loci are explored for human identity testing projects, mitochondrial DNA testing, and…

Q: 1) Using the diagram below, sketch in the pattern of bands you would expect to see after digesting…

A: TAS2R38 is a taste receptor gene present in the 7th chromosome of the genome. The length of a gene…

Q: Why are there multiple results returned when you input a sequence into BLAT or BLAST? How do you…

A: BLAT and BLAST are powerful sequence alignment algorithms used to compare a query sequence to a…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.