You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located? At the 5’ end of the gene and the C-term of your protein of interest At the 5’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the C-term of your protein of interest

Q: You have sequenced the genome of a new E. coli strain that you discovered during the metagenomics…

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: Two homologous chromosomes in ES cells are depicted below with the portion of your gene of interest…

A: CRISPR/Cas9 creates specific double-stranded breaks at the target locus that trigger DNA repair…

Q: The wildtype sequence of a gene is the following: wt:          3' TAC AAA TCT AGC CCG 5'  and the…

A: We'll look at three distinct mutations that were discovered in a wild-type gene sequence in this…

Q: What color is the alpha gene spot on the microarray? Answer: Yellow please explain why is the…

A: The microarray can be described as a lab-on-a-chip. It can be used to detect the expression of…

Q: If a gene contains 2 introns and 3 exons, explain and draw what it would look like in an R loop…

A: R-loop is a structure containing 3 strands of nucleic acid and is all a hybrid of DNA: RNA with…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: Recombination signal sequences are conserved heptamer and nonamer sequences that flank the V, J, and…

A: Introduction: RAG 1 & 2 are the recombination activating genes that encode a portion of a…

Q: 1. a)What would happen if the aminoacyl tRNA synthetase responsible for charging alanine tRNAs also…

A: The polymerization of amino acids according to the codons on mRNA to form proteins is known as…

Q: A method for detecting methylated CpGs involvesthe use of a chemical called bisulfite, which…

A: Hi! Since you have posted multiple questions, we are answering only first three sub-parts. Primers…

Q: recombinant human insulin, produced by bacteria carrying a cloned insulin gene, is now the major…

A: Insulin is an important hormone, which is used for diabetes treatment as it help to reduce the blood…

Q: For a coding gene, purifying (negative) selection will result in more synonymous (silent) site…

A: Purifying selection is the most predominant form of natural selection or negative selection. As it…

Q: You obtained the sequence of the frog gene X you amplified in Question #16 through a process called…

A: A "nucleic acid" is a linear polymer of nucleotides that is a component of the cell's information…

Q: The sequence at one end of one strand of the Drosophilatransposon Mariner is shown below (dots…

A: The genes are the sequence of nucleotides that are present on the chromosomes and encode for a…

Q: Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in…

A: CRISPR-Cas9 is a genome editing tool. It helps researchers to edit or change the gene from the…

Q: It is possible to take the DNA of a gene from any source and place it on a chromosome in the nucleus…

A: Recombinant DNA technology is the process in which the recombination of genetic material takes place…

Q: A very common molecular biology research method is to analyze cell or tissue homogenates by…

A: In molecular biology research, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting,…

Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…

A: A promoter can be defined as a DNA sequence where proteins bind and initiate transcription of an…

Q: Consider a genome whose length is 1000 bp. "Shotgun" sequencing techniques are applied to the…

A: Shotgun sequencing is an important tool in modern genomics that allows researchers to interpret the…

Q: here is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods,…

A: In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or…

Q: You obtain the DNA sequence of a mutant of a 2-kb gene in which you are interested and it shows base…

A: the mutation is the change or error that is caused in the chromosome it is of a different type 1.…

Q: Shown below is an R loop prepared for electron microscopy by annealing a purified eukaryotic…

A: An exon is any part of a gene that will encode a part of the final mature RNA produced by that gene…

Q: A plant geneticist was examining the inversion mutation XI 501 in barley where a segment of DNA is…

A: Any alteration occurring in the DNA sequence of an organism is known as mutation.

Q: ou have sequenced the genome of the bacterium Salmonella typhimurium and find a protein that is 100…

A: Natural selection is a theory that states that organisms evolved by changing their heritable traits…

Q: Some S. aureus strains can produce an enzyme called beta-lactamase, which breaks down beta-lactam…

A: bacteria have dna as their genetic material .

Q: To identify the following types of genetic occurrences, would acomputer program use sequence…

A: Introduction  With the advancements of Bioinformatics tools and sequencing techniques we can easily…

Q: When an EcoR1 fragment, which represents the coding region of a human gene X, is cloned into the…

A: Fusion proteins are created by combining two or more genes into a single gene. GST is a common…

Q: The consensus sequence of the protospacer adjacent motif (PAM) sequence in bacterial strain A is…

A: For Cas9 to work, it additionally requires a particular protospacer adjacent motif (PAM) that…

Q: You design a guide RNA to target your gene of interest, which you will express attached to a…

A: CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 is a genome-editing tool. It…

Q: You know from your calculationsthat only a small proportion of the human genome is represented, even…

A: The objective of the question is to outline a strategy for constructing a genomic DNA library that…

Q: While inspecting the genome sequence of your newly discoyered organism, you note that while the…

A: The GC content of an organism defines the percentage of the nitrogenous bases cytosine (C) and…

Q: DNA sequencing can help us to identify mutations withingenes. The following data are derived from an…

A: Any change in the sequence of DNA is termed as mutation, it can be due to physical or chemical…

Q: In a genomic analysis looking for a specific disease gene,one candidate gene was found to have a…

A: The discovery, measurement, or comparison of genomic properties such as DNA sequence, structural…

Q: DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain…

A: Co transformation means that the two genes are transformed simultaneously. Is two genes are co…

Q: In each of the illustrations below, a segment of a chromosome has two copies of a transposable…

A: The correct answer is: 2. Gene X - Gene Y - TE-A - TE-B - Gene ZHere's why:Homologous Recombination:…

Q: A person with a rare genetic disease has a sample of her chromosomessubjected to in situ…

A: The word insitu derived from the Latin word that means in place. This suggests that the…

Q: Genome annotation refers to ... 1.) lining up overlapping regions in short shotgun sequencing reads…

A: Introduction:- Genome consists of all the genetic and biological information of an individual…

Q: Your colleague wants to align the complete sequence of the collagen gene from humans to the…

A: Homologous genes are genes that share a common ancestry. When two species share a common ancestor,…

Q: Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences…

A: Answer  when a bacteria is infected with bacteriophage is incorporates a small segment into its…

Q: RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with…

A: Biotechnology is the part of applied science that utilizations living organic entities and their…

Q: Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up…

A: Answer. The majority of eukaryotic genes contain non-coding DNA. The genes tend to have a split…

Q: this is what i have said about this image so far, what else can be said aswell including the raw…

A: The raw count data presented in this comprehensive gene expression analysis offers a quantitative…

Q: The following figure shows a screen shot  from the UCSC Genome Browser, focusing  on a region of the…

A: Bioinformatics is the study of biological data through computation, storage, and interpretation.…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.