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If a gene contains 2 introns and 3 exons, explain and draw what it would look like in an R loop experiment.
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- Examine the following sashimi plot from a transcriptomics experiment. The red peaks mostly RNA STAR on data 22; data 16; ar 34 2 1 545326 550237 555148 560059 O a. correspond to exons and represent respective coverage of exons O b. correspond to introns and represent respective coverage of intronsDescribe the outcome of a chain-terminator sequencing procedure in which (a) too little ddNTP is added or (b) too much ddNTP is added.Below is a sequence of 540 bases from a genome. What information would you use to find the beginnings and ends of open reading frames? How many open reading frames can you find in this sequence? Which open reading frame is likely to represent a protein- coding sequence, and why? Which are probably not functioning protein-coding sequences, and why? Note: for simplicitys sake, analyze only this one strand of the DNA double helix, reading from left to right, so you will only be analyzing three of the six reading frames shown in Figure 19.4.
- In studies of frameshift mutations, Crick, Barnett, Brenner, andWatts–Tobin found that either three nucleotide insertions ordeletions restored the correct reading frame. Question: Assuming the code is a triplet, what effect would the addition or loss of six nucleotides have on the reading frame?Assume 2x108 reads of 75 bps long are obtained from a next-generation sequencing experiment to sequence a human genome. Suppose the length of the human genome is 3x109 bps. What is the depth (i.e., coverage) of the sequencing?The practical limit for the number of different RNA sequences screened in a SELEX experiment is 10¹5. Suppose you are working with oligonucleotides 36 nucleotides long. How many sequences exist in a randomized pool containing every sequence possible? total number of sequences: What percentage of these can a SELEX experiment screen? percentage of RNA molecules screened in SELEX experiment: Suppose you wish to select an RNA molecule that catalyzes the hydrolysis of a particular ester. From what you know about catalysis, which SELEX strategy might allow you to select the appropriate catalyst? %
- A molecular geneticist hopes to find a Gene in human liver cell that codes for an important blood-clotting protein,he knows that the nucleotide sequence of a small part of the Gene is GTGGACTGACA.briefly explain how to obtain geneA 2500 bp region of the human genome encodes two genes. One of the genes encodes a protein of 600 amino acids and the other gene encodes a protein of 280 amino acids. The mRNA sequences of the two genes do not contain any of the same nucleotide sequences (i.e. they do not overlap). How is this possible? Fully explain your answer.You obtained the sequence of the frog gene X you amplified in Question #16 through a process called automated sequencing. In automated sequencing, you are given a printout of the sense strand of your DNA. The printout is shown below. The first thing you need to do is use the correct reading frame. Having done this, the next thing to do is to write out the mRNA sequence using this sense strand reading frame. The last thing to do is to translate the sequence. Do these steps in the space below. The reading frame DNA sequence is: 2.The mRNA sequence is: The polypeptide sequence is:
- A molecular geneticist hopes to find a gene gene in human liver cells that codes for an important blood clotting protein. He knows that the nucleotides sequence of a small part of the gene is GTGGACTGACA. briefly explain how to obtain the desired geneWatch the following video called "Human Genome Project Video" (https://fod.infobase.com/OnDemandEmbed.aspx?lti=1&token=30037&wID=97629&loid=0&w=400&h=300) then answer the following question in one paragraph each with evidence from the film to support your answers. 1) In one paragraph, explain in detail the purpose of decoding the entire human genome according to what you have seen from the provided link video, read online or read from the book. 2) In one paragraph, explain how has decoding the human genome been used in medicine? Give a couple of examples from the provided video link and provide a detailed explanation.Suppose you had funding to do a genome-wide gene expression experiment (20,000 genes). You can use a total of 20 RNA samples. Briefly describe the experiment you would run. In the context of your experiment, what is a false positive? In the context of your experiment, what is a false negative
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