For each of the following scenarios describing gene annotation, choose the answer that bestfitsA researcher searches a database ofsequence motifs for matches to parts of agene's sequence.A researcher searches for a genomicregion that includes particularcharacteristics that include the following:a CpG island near a promoter, a TATA boxand 5 and 3' splice sites.A researcher identifies genes based onknown expression.Chooseab initio annotation in a eukaryotic genomeab initio annotation in a prokaryotic genomehomology-based annotationab initio annotation to investigate a gene's function

Answered: Image /qna-images/answer/8b0c6334-0fd3-4595-ac1b-b543e6b4c7b1.jpg

Answered: For each of the following scenarios…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The entire genome of the yeast Saccharomyces cerevisiaehas been sequenced. This sequencing has led to the identification of all the open reading frames (ORFs, gene-sizesequences with appropriate translational initiation andtermination signals) in the genome. Some of these ORFsare previously known genes with established functions;however, the remainder are unassigned reading frames(URFs). To deduce the possible functions of the URFs,they are being systematically, one at a time, convertedinto null alleles by in vitro knockout techniques. The results are as follows:15 percent are lethal when knocked out.25 percent show some mutant phenotype (alteredmorphology, altered nutrition, and so forth).60 percent show no detectable mutant phenotype at alland resemble wild type.Explain the possible molecular-genetic basis of thesethree mutant categories, inventing examples wherepossible.
There is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods, steps, and key substances you need to obtain to use the following techniques in experimental design to study the gene: - In situ hybridization (to find the mRNA) - Immunohistochemistry (to find the protein) - CRISPR-Cas9 (for loss of function) - Expression vector (for gain of function)
Suppose a researcher previously cloned gene Y into M13 bacteriophage vector. Gene Y encodes a product called peptide Y. A region of gene Y contains the DNA sequence ATG-CGC-GAA-CTG-GTG-AAC-TAA. The researcher wishes to change a Val residue to an Ala residue in this region of peptide Y using site-directed mutagenesis. What should be the sequence of the mutant oligonucleotide primer in this region? You may use a codon table. mutant oligonucleotide primer sequence: GGC-GGC-GAA-CTG-GTG-AAC-TAA Incorrect
The text has discussed the RNA-Seq technique, whichquantifies the amounts of mRNAs present in a given celltype at a given time. We have also described the methodof ribosome profiling, which quantifies the levels ofmRNA sequences that are being translated actively in acell population at a given time. But even when bothkinds of experiments are performed, the data do not tellus accurately the relative amounts of the protein products of these mRNAs in the cells at that time. Why not?What elements of gene expression do these techniquesaccount for and what elements do they ignore?
the topic is Identification of Differentially Expressed Genes in Breast Cancer Using RNA-Seq write a method section of a scientific report for this topic. including the use of excel to get these result( will be included in the images) and also this website https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE216238
siRNA and CRISPR/Cas9 each interfere with gene expression by a sequence-directed mechanism. A. Identify the target macromolecule (DNA, RNA or Protein) of a specific siRNA/RISC andCRISPR/Cas9 complexsiRNA:CRISPR/Cas9: B. If a scientist would like to permanently turn off expression of a particular human gene inthe liver for the lifetime of an individual, which of these techniques would bepreferable? Briefly explain why
a) The best vector to use determine receptor binding protein expression would have been one with a GFP gene (green flourescent protein) attached. State one (1) reason why including this gene would have made the experiment easier and whether you have inserted the receptor binding domain gene before or after the GFP gene. b) You wish to determine the sucess pf your transformation by detecting the presence of receptor binding domian mRNA. Describe the key steps of the hybrdization technique you would use, clearly stating how you would design the probe to detect your receptor mRNA.
An electrophoretic mobility shift assay can be used to study the bindingof proteins to a segment of DNA. In the results shown here, anelectrophoretic mobility shift assay was used to examine the requirementsfor the binding of RNA polymerase II (from eukaryotic cells)to the promoter of a protein-encoding gene. The assembly of generaltranscription factors and RNA polymerase II at the core promoteris described in Chapter 14 (Figure 14.14). In this experiment, thesegment of DNA containing a promoter sequence was 1100 bpin length. The fragment was mixed with various combinations ofproteinsand then subjected to an electrophoretic mobility shift assay.Explain which proteins (TFIID, TFIIB, or RNA polymerase II) areable to bind to this DNA fragment by themselves. Which transcriptionfactors (i.e., TFIID or TFIIB) are needed for the binding ofRNA polymerase II?

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