Strand Length TONSL vs Primary Description foldchange log2FC PValue log2FPKM Raw counts FDR 5812 EYA transcri 3604.4176 11.81555 2.85E-284 5847 polypeptide 1099.1348 10.102153 4.99E-95 2717 ISL LIM hom 847.28604 9.7267053 1.49E-77 2823 inositol hexa 791.81993 9.6290286 8.59E-75 1078 ZFPM2 antis 726.06516 9.5039552 2.36E-69 2228 p53-induced 608.11897 9.2482098 1.2E-267 4983 hippocalcin 456.80467 8.8354336 4.56E-44 6846 cut like hom 384.39701 8.5864533 3.782E-39 3747 lipoprotein li 367.82505 8.5228759 2.41E-37 4132 neurotensin 346.01397 8.4346865 1.09E-117 3036 homeobox B 299.68622 8.2273089 4.24E-31 838 fatty acid bir 278.67084 8.1224182 3.13E-94 2805 carboxypept 277.74252 8.1176043 1.11E-252 3126 SHC binding 259.38227 8.0189361 1.16E-26 2465 SAM domain 239.98475 7.9067989 4.70E-123 6698 uncharacter 238.77865 7.89953 1.36E-24 785 colony stimu 236.26536 7.8842643 5.56E-25 3564 SPARC (osted 229.13316 7.8400424 2.31E-77 5354 dihydropyrin 226.52461 7.823524 1.01E-23 3496 coiled-coil do 220.17798 7.7825264 5.61E-134 397 metallothion 219.35572 7.7771285 7.71E-23 2785 gamma-ami 217.6387 7.7657913 4.81E-22 8527 neural cell a 215.16056 7.7492699 6.93E-23 9270 janus kinase 212.64257 7.7322866 1.62E-22 2795 colon cancer 197.98603 7.6292548 1.16E-192 5426 retrotranspo 194.05707 7.6003372 9.75E-21 2231 vestigial like 184.90298 7.5306247 2.024E-19 835 long interge 184.50034 7.5274797 5.48E-20 7477 NACHT and 176.87488 7.4665854 9.81E-60 4680 meiosis spec 168.78924 7.3990791 2.12E-17 3389 long interge 167.96708 7.3920347 2.505E-18 3429 coagulation 164.74515 7.3640922 5412 cilia and flag 162.17956 7.3414482 642 ADP ribosyla 161.14218 7.3321904 323 cancer susce 154.37541 7.2702992 KTB_103_pr KTB103_prir KTB103_prir KTB_103_TC KTB_103_TC KTB_103_TCKTB_103_pr KTB103_prir KTB103_prir KTB_103_TC KTB_103_TC KTB_103_TONSL-F_S22 1.55E-282 -9.3129082 -9.3129082 -9.3129082 1.4648813 1.5160533 1.5272297 0 0 0 500 557 5.49E-94 -9.32157 -9.32157 -9.32157 -0.0733843 -0.3162863 -0.2790863 1.33E-76 -8.215888 -8.215888 -8.215888 0.631853 0.3966124 0.4940631 7.38E-74 -8.2711026 -8.2711026 -8.2711026 0.4262034 0.3831127 0.2707696 1.87E-68 -6.8822306 -6.8822306 -6.8822306 1.5912286 1.7160924 1.5676387 5.95E-266-7.9296227 -4.7142693 -7.9296227 2.9934394 3.2481759 3.2192825 2.38E-43 -9.090888 -9.090888 -9.090888 -1.1241791 -1.4468333 -1.2171597 1.792E-38 -9.5491347 -9.5491347 -9.5491347 -1.975471 -1.9613751 -1.9412599 1.10E-36 -8.6796094 -8.6796094 -8.6796094 -1.0796112 -1.2430199 -1.1466266 1.53E-116 -6.2563842 -8.8207137 -8.8207137 0.5240714 0.6412797 0.7507498 1.66E-30 -8.3760452 -8.3760452 -8.3760452 -1.1290682 -1.181848 -1.125163 3.41E-93 -6.5188956 -6.5188956 -3.9634506 2.7864228 2.5182696 2.5766006 4.89E-251 -4.824966 -5.8989967 -5.7064293 2.602651 2.6864677 2.481924 4.07E-26 -8.4181912 -8.4181912 -8.4181912 -1.4660267 -1.5624062 -1.1988073 6.92E-122 -8.0754611 -4.8601077 -8.0754611 1.840504 1.6763788 1.4771684 4.48E-24 -9.5176038 -9.5176038 -9.5176038 -2.8844576 -2.4008619 -2.578528 1.86E-24 -6.424638 -6.424638 -6.424638 0.3993354 0.5234777 0.475236 2.06E-76 -8.6073708 -8.6073708 -6.0519258 0.1260692 0.4506639 0.2011305 3.26E-23 -9.1944906 -9.1944906 -9.1944906 -2.326509 -2.5992509 -2.21725 9.24E-133 -8.5795786 -8.5795786 -5.1523782 1.0913564 1.0728451 0.9667178 2.43E-22 -5.4410844 -5.4410844 -5.4410844 1.382889 1.035158 1.5361562 1.49E-21 -8.2515508 -8.2515508 -8.2515508 -1.1444625 -1.5463855 -1.8165229 2.19E-22 -9.8659117 -9.8659117 -9.8659117 -3.0419383 -3.0586068 -3.2266579 5.07E-22 -9.9864428 -9.9864428 -9.9864428 -3.0757558 -3.3912031 -3.3003502 3.13E-191 -5.6923922 -4.5091346 -8.2567217 2.0109594 2.2237047 2.0842599 2.88E-20 -9.2137625 -9.2137625 -9.2137625 -2.7423719 -2.4064576 -2.6730109 5.761E-19 -7.931564 -7.931564 -7.931564 -1.2482929 -1.1744252 -1.8006939 0 0 0 173 0 0 0 131 160 127 0 0 0 118 113 0 0 0 101 106 0 2 0 553 690 0 0 0 71 0 0 0 54 0 0 0 55 1 0 0 185 231 0 0 0 43 0 0 1 180 166 2 1 1 531 521 0 0 0 35 0 2 0 275 228 0 0 0 28 0 0 0 32 0 0 1 121 0 0 0 33 0 0 2 232 0 0 0 32 0 0 0 39 0 0 0 32 0 0 0 34 1 3 0 351 0 0 0 25 0 0 0 29 4.42E-80 5.75E-16 2.854E-17 1.68E-16 1.59E-19 -6.5137216 -6.5137216 -6.5137216 0.0674955 0.023246 -0.0248636 6.66E-59 -7.1120034 -9.676333 -9.676333 -1.4341127 -1.0221177 5.66E-17 -9.000382 -9.000382 -9.000382 -2.5289914 -2.1930771 -3.1695305 6.881E-18 -8.5347331 -8.5347331 -8.5347331 -2.0073844 -2.257696 -2.155622 4.09E-79 -5.9873318 -6.1887839 -8.5516614 0.9126174 0.3763727 0.6116177 1.46E-15 -9.2100353 -9.2100353 -9.2100353 -3.3733841 -3.2502828 -2.2709594 7.575E-17 -6.1345187 -6.1345187 -6.1345187 -0.2118904 0.5167305 0.2445925 0 0 0 27 -1.109217 1 0 0 86 0 0 0 25 0 0 0 26 1 1 0 201 0 0 0 16 0 0 0 17 4.36E-16 -5.1434795 -5.1434795 -5.1434795 1.1457019 1.5077697 0.6873719 0 0 0 22 322222222222222222 136 227 394 127 174 ཥ ཥེ ཀྐ 8 8 ང ཆ 8ཀྐུཊྛཝ8 8 བྷ ཀྐུ ཙྪཱུ སྐུ ཊུ➢g➢མཎྜ»བཌ 71 59 56 46 45 37 36 38 38 26 30 31 28 21 27 115 17 25 37 25 17 GeneSymbol Chr EYA4 GALNT13 ISL1 IP6K3 HPCAL4 CUX2 LPL NTSR1 HOXB13 FABP4 CPVL Gene information End Start chr6;chr6;ch 133241357;: 133241749;:- chr2;chr2;ch 153871898;: 153872303; chr5;chr5;ch 51383124;5:51383699;5: chr6;chr6;ch 33721666;3:33723187;3: ZFPM2-AS1 chr8;chr8;ch 105780410;: 105780567;:- PINCR chrX;chrX;ch 43176994;4:43177205;4: +;+ chr1;chr1;ch 39678648;3:39682733;3 chr12;chr12 111034024;: 111034240;: chr8;chr8;ch 19939071;1: 19939528;1- chr20;chr20 62708837;6:62709921;6: +;+;+;+ chr17;chr17 48724765;448727043;4(-;- chr8;chr8;ch 81478497;8: 81478915;8:---- chr7;chr7;ch 28995231;228995882;21-;-;-;-) LOC1004205 chr19;chr19; 28435389;228436815;2-;-;-;- SAMSN1 chr21;chr21; 14485228;1414486114;14- LOC642366 chr5;chr5 51372737;5:51379075;5:-;- CSF2 SPOCK3 DPYSL5 CCDC178 MT1A GABRA3 NCAM1 JAKMIP2 CCAT1 RTL9 VGLL2 chr5;chr5;ch 132073792;: 132073982; +;+;+;+ chr4;chr4;ch 166733385;: 166735090;-;-; chr2;chr2;ch 26848101;2(26848254;2( +;- chr18;chr18 32937402;3:32938091;31--- chr16;chr16:56638666;5(56638766;5(+;+;+ chrX;chrX;ch 152167162;:152168563;-;-;- chr11;chr11 112961247; 112961664; chr5;chr5;ch 147585441;: 147591686;: chr8;chr8 127207382;:127209717;-;- chrX;chrX;ch 110419057;: 110419135;: +;+;+;+ chr6;chr6;ch 117265558;: 117265844;: +;+;+; LINC01704 chr1;chr1;ch 158132044;: 158132656;-;- NWD2 chr4;chr4;ch 37245068;3137245218;31 MEIOC chr17;chr17 44656394;4444656682;44 +;+ LINC00299 chr2;chr2;ch 8007771;80( 8008759;80( F2RL2 CFAP74 ARL14EPL CASC19 chr5;chr5;ch 76615482;7(76618642;7(-;-;-;- chr1;chr1;ch 1921951;19: 1922388;191----- chr5;chr5;ch 116051466;: 116051561;:+;+;+ chr8;chr8;ch 127187786;: 127187884;-;-;-
this is what i have said about this image so far, what else can be said aswell including the raw count column.
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Interpreting the results of an RNA-Seq analysis is pivotal in understanding the underlying genetic mechanisms of diseases such as breast cancer. In this analysis, Figure 1 provides comprehensive data on differentially expressed genes associated with breast cancer. By delving into the provided information, we can gain valuable insights into the molecular landscape of this disease. First focus is on the gene with the highest fold change, EYA4, situated on chromosome 6. With a staggering fold change of 3604.4176, EYA4 exhibits an unprecedented level of overexpression in cancerous cells compared to normal cells. This profound alteration suggests a pivotal role for EYA4 in breast cancer pathogenesis. The log2 fold change of 11.81555 further emphasizes the magnitude of this difference in gene expression. Statistical significance is evident, with an exceptionally low p-value of 2.85E-284, indicating a highly improbable occurrence under the null hypothesis. Moreover, the false discovery rate (FDR) of 1.55E-282 reinforces the robustness of this finding, signifying minimal chances of a false positive result. Continuing with the examination of the gene with the second highest fold change, GALNT13, located on chromosome 2, one observes a fold change of 1099.1348. Although not as pronounced as EYA4, GALNT13 still exhibits substantial dysregulation in cancer cells. The log2 fold change of 10.102153 underlines the significant difference in expression levels between normal and cancerous tissues. This observation is further supported by a low p-value of 4.99E-95, indicating statistical significance, and an FDR of 5.49 E-94, which reinforces the reliability of the result.
Expanding the analysis to include other genes with notable fold changes, a spectrum of dysregulated genes implicated in breast cancer progression emerges. GABRA3, NCAM1, ISL1, IP6K3, PINCR, ZFPM2-AS1, HPCAL4, CUX2, LPL, NTSR1, HOXB13, FABP4, CPVL, LOC1004205, SAMSN1, LOC642366, CSF2, SPOCK3, DPYSL5, CCDC178, and MT1A all exhibit significant alterations in expression levels, with fold changes ranging from 215.16056 to 847.28604. These findings underscore the multifactorial nature of breast cancer, implicating a diverse array of genes in its pathogenesis."
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