Can you please answer number 4 overexpressed in nervous tissue cancers, breastcancers, and soft tissue tumors at a frequencyof 10-25%. Obtain the sequence of the humanparalog MDM4 and the mouse paralog MDM4 andperform multiple sequence alignment togetherwith the original five MDM2 sequences listedin problem 2. Give amino acid ranges of thedomains (in human MDM2 amino acid numbers)that are highly conserved within sequences ofthis entire homolog family.4. The quagga is an animal indigenous to Africa thatis now extinct. It looked partly like a donkey andpartly like a zebra. In 1872, the last living quaggawas photographed. Mitochondrial DNA was ob-tained from a museum quagga specimen andsequenced.a. Perform a BLAST search with the quagga (Equusburchellii quagga) mitochondrial 125 ribosomalDNA query and find out if the quagga is moreclosely related to the donkey (Equus asinus) or thezebra (Equus zebra). Support your answer with data.b. Perform a Clustal analysis of mitochondria12S ribosomal DNA sequences from the threespecies. Along with the outputted multiplesequence alignment an identity matrix is created.Use the identity matrix to confirm the conclusionyou made from the BLAST analysis in part a.

Quagga: previously present in large herds on the immense plains of southern Africa, but now extinct. The color of the head and neck top regions of the body was a reddish brown, unevenly banded, and marked with dark brown stripes, stronger on the head and neck and gradually becoming fainter until lost behind the shoulder. A large, black dorsal median stripe was seen. The underside of the torso, legs, and tail were almost completely white and striped-free. The crest was tall and unstriped. A standing mane with brown and white bands topped the high crest. The last quagga to be found perished at the London zoo in 1872. To determine whether DNA survives and can be recovered from the remains of extinct creatures, we have examined dried muscle from a museum specimen of quagga a zebra-like species (Eqqus quagga) that become extinct in 1883. We report that DNA was extracted from the tissue in amounts approaching 1% of that expected from fresh muscle, and that the DNA was of relatively low molecular weight. Among many clones obtained from the quagga DNA two containing pieces of mitochondrial DNA were sequenced. These sequences comprising 229 nucleotides pairs, differ by 12 base substitutions from the corresponding Sequence of mtDNA from a mountain zebra an extinct member of genus Equus. The number nature has been little** No postmortem modification of the Quagga DNA sequences, and that the two species had a common ancestor 3-4 Myr ago, consistent with fossil evidence concerning the age of the genus Equus. The DNA sequence of quagga showed it was more related to zebras.*little is known about quagga behavior but it may have gathered into herds of 30-50 . Quaggas were said to be wild or lively. Yet was considered more docile than the related Burchell's zebra. They were once found in great numbers in karoo of cape Sothern part of orange free state. The last captive specimen died in Amsterdam on 12 august 1883. Only one quagga was ever photographed alive and only 23 skins exist today. In 1984, the quagga was first extinct animal whose DNA was analyzed.Clustal omega is a general purpose multiple sequence alignment program for DNA or proteins. clustal omega is a new multiple sequence alignment program that uses seeded guide trees and HMM profilr techniques to generate alignments between 3 or more sequences. For the alignment of 2 sequences use pairwise sequence alignment tools