In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true: I. Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype II. Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type) III. Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant
I. |
Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype |
|
II. |
Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type) |
|
III. |
Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype |
|
IV. |
Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to white |
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Wild type
white
rosy
5' P-element end
transposase
inverted repeats
Syncytium
injection
Helper
3' P-element end 5' P-element end
B
white
experimental Selection marker
inverted repeats
Germline
Nucleus
transposase
3' P-element end
Genome"
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