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This question relates to the biological activity of the estrogen receptor (ER). ER is a nuclear receptor and positively regulates the expression the c-Myc gene, a transcription factor found at elevated levels in numerous cancers. To understand the effect of drugs on the biological behaviour of the estrogen receptor in cells, you undertake Western Blotting analysis of a cell line that expresses a recombinant human estrogen receptor. After culturing the cells, you then lyse the cells and fractionate them, generating membrane, cytosolic and nuclear protein fractions from the whole cell lysate. After running these fractions on SDS-PAGE gels, you transfer to nitrocellulose membrane and probe with monoclonal antibody conjugates for the proteins ER and c-Myc. The results of your Western Blotting experiment without any treatment are provided in Figure 1.

This question relates to the biological activity of the estrogen receptor (ER). ER is a nuclear receptor and positively regulates the expression the c-Myc gene, a transcription factor found at elevated levels in numerous cancers. To understand the effect of drugs on the biological behaviour of the estrogen receptor in cells, you undertake Western Blotting analysis of a cell line that expresses a recombinant human estrogen receptor. After culturing the cells, you then lyse the cells and fractionate them, generating membrane, cytosolic and nuclear protein fractions from the whole cell lysate. After running these fractions on SDS-PAGE gels, you transfer to nitrocellulose membrane and probe with monoclonal antibody conjugates for the proteins ER and c-Myc. The results of your Western Blotting experiment without any treatment are provided in Figure 1.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Using your Western Blot data and your knowledge of nuclear receptors, discuss which type of drug you would recommend for a cancer therapy targeting c-Myc expression.

This question relates to the biological activity of the estrogen receptor (ER). ER is a nuclear receptor and positively regulates the expression the c-Myc gene, a transcription factor found at elevated levels in numerous cancers. To understand the effect of drugs on the biological behaviour of the estrogen receptor in cells, you undertake Western Blotting analysis of a cell line that expresses a recombinant human estrogen receptor. After culturing the cells, you then lyse the cells and fractionate them, generating membrane, cytosolic and nuclear protein fractions from the whole cell lysate. After running these fractions on SDS-PAGE gels, you transfer to nitrocellulose membrane and probe with monoclonal antibody conjugates for the proteins ER and c-Myc. The results of your Western Blotting experiment without any treatment are provided in Figure 1. Primary antibody Cell fraction Anti-ER Anti-c-myc Membrane Cytoplasm Nucleus | |
Transcribed Image Text:This question relates to the biological activity of the estrogen receptor (ER). ER is a nuclear receptor and positively regulates the expression the c-Myc gene, a transcription factor found at elevated levels in numerous cancers. To understand the effect of drugs on the biological behaviour of the estrogen receptor in cells, you undertake Western Blotting analysis of a cell line that expresses a recombinant human estrogen receptor. After culturing the cells, you then lyse the cells and fractionate them, generating membrane, cytosolic and nuclear protein fractions from the whole cell lysate. After running these fractions on SDS-PAGE gels, you transfer to nitrocellulose membrane and probe with monoclonal antibody conjugates for the proteins ER and c-Myc. The results of your Western Blotting experiment without any treatment are provided in Figure 1. Primary antibody Cell fraction Anti-ER Anti-c-myc Membrane Cytoplasm Nucleus | |
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